Application of rapamycin in the treatment of early brain injury of subarachnoid hemorrhage
A subarachnoid, rapamycin technology, applied in cardiovascular system diseases, nervous system diseases, drug combinations, etc., to achieve the effects of enhancing autophagy, reducing EBI, and reducing cerebral edema
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[0030] 1. Materials:
[0031] 1.1 Reagents and experimental equipment
[0032] 1.1.1 Main Drugs and Reagents
[0033] Rapamycin (RAP) (purchased from Sigma-aldrich, USA), 3-MA (purchased from Sigma-aldrich, USA), Evans blue (purchased from Shanghai Baoman Biotechnology Co., Ltd.), 50% trichloroacetic acid ( TCA), absolute ethanol, etc.
[0034] 1.1.2 Main Instruments
[0035] Electronic scale (FA2004B Shanghai Yueping Technology Instrument Co., Ltd.), oven (DHG9070B Shanghai Langgan Experimental Equipment Co., Ltd.), homogenizer (985-370 BIOSPEC PRODUCTS, INC), centrifuge (GL-16A Shanghai Fischer Analytical Instrument Co., Ltd. company), spectrophotometer (UV-757 Shanghai Mapoda Instrument Co., Ltd.), stereotaxic instrument (ALCB10 Shanghai Alcott Biotechnology Co., Ltd.), 5-μL micro sample injector (Shanghai Gaoge Industry and Trade Co., Ltd. )Wait
[0036] 1.2 Experimental animals
[0037] Adult healthy male Sprague-Dawley (S-D) rats were provided by the Experimental A...
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