A method for extracting propolis containing high-purity flavonoids
An extraction method and technology of flavonoids, applied in the field of propolis extraction containing high-purity flavonoids, can solve the problems of undiscovered flavonoids, complicated operation, low quality, etc. safe effect
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preparation example Construction
[0317] One of the preparation methods of propolis extract:
[0318] (1) Preparation of propolis extract crude product
[0319] The propolis raw material is heated and impregnated with 95% ethanol, heated and decolorized with activated carbon + activated clay, cooled, filtered or centrifuged, the supernatant is concentrated and recovered, spray-dried and pulverized to obtain a crude propolis extract;
[0320] (2) Refined propolis extract
[0321] Dissolve the crude product of propolis extract with ethanol, add hydrochloric acid to hydrolyze it to obtain flavonoid aglycone, concentrate to extract, dissolve the extract with more than 80% ethanol, filter or centrifuge, concentrate and recover the supernatant, spray dry and pulverize, and obtain refined propolis extract;
[0322] The preparation method two of propolis extract:
[0323] (1) Preparation of propolis extract crude product
[0324] The propolis raw material is heated and impregnated with 75% ethanol, after ultrasoni...
Embodiment 1
[0340] (1) Preparation of propolis extract crude product
[0341] 1000g of propolis raw material was heated and impregnated with 75% ethanol, after ultrasonic treatment, allowed to cool, filtered or centrifuged, the supernatant was concentrated and recovered, spray-dried and pulverized to obtain 445g of crude propolis extract;
[0342] (2) Refined propolis extract
[0343] Dissolve 445g of propolis extract crude product in 95% ethanol, add hydrochloric acid to hydrolyze to obtain flavonoid aglycone, concentrate to extract, extract extract is dissolved in more than 80% ethanol, filter or centrifuge, concentrate and recover the supernatant, spray dry and pulverize , obtain refined propolis extract 293g, flavonoid content 9.2%, lead content 0.013mg / Kg.
Embodiment 2
[0345] (1) Preparation of propolis extract crude product
[0346] 1000g of propolis raw material was heated and impregnated with 85% ethanol, after microwave treatment, allowed to cool, filtered or centrifuged, the supernatant was concentrated and recovered, spray-dried and pulverized to obtain 456g of crude propolis extract;
[0347] (2) Refined propolis extract
[0348] Dissolve 456g of crude propolis extract in 95% ethanol, hydrolyze with hydrochloric acid to obtain flavonoid aglycone, concentrate to the extract, dissolve the extract in more than 80% ethanol, filter or centrifuge, concentrate and recover the supernatant, spray dry and pulverize , obtain refined propolis extract 310g, flavonoid content 9.8%, lead content 0.014mg / Kg.
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