Immobilized laccase and preparation method thereof

Abstract

The invention discloses an immobilized laccase, which adopts magnetic mesoporous carbon as a vector, the laccase is immobilized on the magnetic mesoporous carbon by means of the physical adsorption effect, magnetic nanoparticles are embedded in the pore canals of the magnetic mesoporous carbon, the amount of the laccase adsorpted on the magnetic mesoporous carbon is more than 140mg / g, and the enzyme activity recovery rate of the immobilized laccase is 60 to 95 percent. The invention correspondingly discloses a preparation method for the immobilized laccase, which includes the following steps:the magnetic mesoporous carbon and laccase solution are first prepared, the concentration of the laccase solution is 0.3mg / ml to 1.0mg / ml, and the pH value of the laccase solution is 3.0 to 6.0; the magnetic mesoporous carbon is then added into the laccase solution, and the solution is oscillated under the temperature of 25 DEG C to 30 DEG C for more than three hours; and after subsequent processing, the immobilized laccase is obtained. Since the immobilized laccase disclosed by the invention is immobilized on the basis of the magnetic mesoporous carbon, the laccase has excellent enzymological properties; and the preparation method for the immobilized laccase is easy to operate, and the cost is low.

Description

technical field [0001] The invention relates to the field of enzyme immobilization, in particular to an immobilized laccase and a preparation method thereof. Background technique [0002] Laccase (laccase, E.C.1.10.3.2) is a copper-containing polyphenol oxidase widely found in fungi, bacteria and plants. Laccase has a wide range of substrate specificity and strong stability, and can catalyze many phenols and aromatic amines, such as phenol, p-chlorophenol, aniline, and polyphenols and polyamines. , biofuel cells, biotransformation and wastewater treatment and other fields have a wide range of applications. However, since laccase is easily soluble in water, the free laccase in water cannot be recovered or stored for a long time, and the stability of laccase is not easy to maintain under high temperature or other extreme environments, thus limiting the practical application of laccase. Immobilization technology not only maintains the specific and efficient catalytic activity...

AI Technical Summary

Problems solved by technology

It has been reported in the literature that immobilizing laccase with magnetic mesoporous silicon with a large pore size has a higher activity recovery rate of the immobilized enzyme, and improves the pH stability and thermal stability of the laccase (Yufang Zhu et al, Chemistry of Materials, 2007 , 6408-6413), but this kind of mesoporous material has the disadvantages of cumbersome preparation process and difficult separation.

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