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New vascular tissue generation method

An angiogenesis and tissue technology, applied in the direction of vertebrate cells, artificial cell constructs, animal cells, etc., can solve the problems of low survival rate and cumbersome quantification, and achieve the effect of overcoming the low survival rate and cumbersome quantification

Inactive Publication Date: 2012-01-18
XI AN JIAOTONG UNIV
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Problems solved by technology

[0004] In order to overcome the deficiencies in the above-mentioned prior art, the object of the present invention is to provide a new tissue angiogenesis method, which overcomes the shortcomings of cumbersome quantification and low survival rate, and establishes a simple, convenient and widely applicable method. A model of angiogenesis in new tissues, this method can also be applied to the screening of inhibitors of tumor angiogenesis

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Embodiment Construction

[0014] The present invention will be described in more detail below in conjunction with the accompanying drawings and embodiments.

[0015] New tissue angiogenesis method, take mouse or rat thoracic aorta, heart, liver, spleen, lung, kidney and colon tissue under sterile conditions, then put them into pre-cooled PBS solution for cleaning, thoracic aorta processing method In order to remove the fibrous and fatty tissue outside the arterial tube with ophthalmic surgical scissors and forceps, after washing with PBS solution, under a magnifying glass, cut the arterial ring with a length of 0.3 mm or cut it into a length of 0.3 mm in pre-cooled DMEM medium. mm arterial block, and heart, liver, spleen, lung, blood vessel, kidney and colon tissue are processed by using ophthalmic surgical scissors and forceps to remove the adhesion tissue around the tissue and cut it into 1mm 3 For tissue pieces of different sizes, take the fibrinogen solution and spread it in a 48-well culture plate...

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Abstract

The invention relates to a new vascular tissue generation method. The method comprises, removing thoracic aorta, heart tissue, liver tissue, spleen tissue, lung tissue, kidney tissue and colon tissue from a mouse or a rat under a sterile condition; carrying out a combination of the removed tissues and the culture plate to generate the new vascular tissues respectively corresponding to the aorta, the heart tissue, the liver tissue, the spleen tissue, the lung tissue, the kidney tissue and the colon tissue. With the method provided by the present invention, disadvantages of cumbersome quantity and not high survival rate are overcome; a simple and convenient model of the new vascular tissue generating is established, wherein the model can be widely used; the method further can be applicable for screening studies of the tumor angiogenesis inhibitor.

Description

technical field [0001] The invention belongs to the field of compound pharmacological action and molecular biology technology, and specifically relates to a new tissue angiogenesis method. Background technique [0002] Angiogenesis refers to the regeneration of new blood vessels on the basis of the original vascular bed. It is a complex process regulated by multiple factors, which is different from the process of angiogenesis. Angiogenesis is a dynamic and coordinated complex process involving a variety of cytokines and cellular components. The central link in its initiation is the migration, division, and differentiation of vascular endothelial cells or stromal stem cells, and the subsequent luminal is regulated by angiogenesis inducers and inhibitors. Physiological angiogenesis is mostly seen in wound healing, or angiogenesis in the endometrium during the menstrual cycle, but it is a part of some processes such as tumors, arteriosclerosis, psoriasis, diabetic retinopathy,...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 张彦民贺浪冲代秉玲王楠郑蕾王嗣岑张杰
Owner XI AN JIAOTONG UNIV
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