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RAPD-SCAR839 label for identifying group E chromosome of thinopyrum elongatum and application of same

A technology of E. elongatum and E. elongatum, applied in the field of E group chromosome identification of E. elongatum, can solve the problems of poor repeatability and stability, and achieve the effect of improving accuracy and correct rate

Inactive Publication Date: 2012-03-14
HARBIN NORMAL UNIVERSITY
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Problems solved by technology

However, its repeatability and stability are poor. If it is converted into a SCAR marker, its specificity and stability will be greatly improved, and it can be more conveniently and quickly applied to the detection of heterologous chromosomes

Method used

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  • RAPD-SCAR839 label for identifying group E chromosome of thinopyrum elongatum and application of same
  • RAPD-SCAR839 label for identifying group E chromosome of thinopyrum elongatum and application of same
  • RAPD-SCAR839 label for identifying group E chromosome of thinopyrum elongatum and application of same

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Embodiment Construction

[0027] The invention provides a RAPD-SCAR for identifying E group chromosomes of E. elongatum 839 Markers and their applications, the specific RAPD fragments were screened by the RAPD-SCAR method, converted into stable SCAR markers, and their amplification was detected. The preparation, amplification and identification of markers will be further described in detail in conjunction with specific examples below, which are explanations rather than limitations of the present invention.

[0028] Sources of material involved:

[0029] "Chinese Spring" wheat: It comes from the Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Harbin Normal University. In April 2010, the material was planted in the Key Experimental Nursery Base of Molecular Cytogenetics and Genetic Breeding of Harbin Normal University. DNA was extracted 2 months later. Molecular marker screening assay.

[0030] Diploid E. elongatum: sourced from the Wheat Center of the National Bioresources Program of ...

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Abstract

The invention discloses an RAPD-SCAR83 label for identifying group E chromosome of thinopyrum elongatum and application of the same. A nucleotide sequence of the group E chromosome is as shown in SEQ. ID. NO. 2 or SEQ. ID. NO. 5. In the invention, specific RAPD segments are screened to obtain through an RAPD-SCAR method, and are converted into stable SCAR labels; and amplification of the RAPD labels is detected. When the label of the group E chromosome of the thinopyrum elongatum provided by the invention is adopted, a solid foundation is laid for quickly identifying a 2E or 3E chromatin or chromosome of the thinopyrum elongatum under the genetical background of wheat through the detection of the SCAR labels, and a very convenient and quick means is also provided for molecular marker-assisted breeding.

Description

technical field [0001] The invention belongs to the technical field of identification of E group chromosomes of E. elongatum, and relates to a RAPD-SCAR for identifying E group chromosomes of E. elongatum 839 Marking and its application. Background technique [0002] Wheat is one of the important food crops in the world. In China, it is the second largest crop after rice, and wheat production is of great strategic significance to the development of the national economy. Due to the simplification of wheat cultivars, its genetic base is increasingly narrow and its resistance sources are scarce, which greatly limits the further improvement of its yield and quality. There are a large number of genetic variations in close relatives of wheat, which are beneficial gene resources that can be used for wheat improvement. The introduction of these beneficial genes into wheat has always been an enduring research topic for geneticists and breeders. Enopyrum is a close relative of whea...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 郭长虹徐国辉姜格格王丹孙媛媛赵迪吴磊
Owner HARBIN NORMAL UNIVERSITY
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