Anti-tumor and thrombolytic dual-effect chimeric protein with low immunogenicity, its preparation method and application

A chimeric protein, immunogenic technology, applied in the field of microbiology, can solve the problems of single, tumor complicated by thrombus without specific drugs, etc., and achieve the effect of reducing toxic side effects, low immunogenicity, and small molecular weight
CN102492043BActive Publication Date: 2013-09-18LIAONING UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LIAONING UNIVERSITY
Publication Date
2013-09-18

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Abstract

The invention relates to an anti-tumor and thrombolytic dual-effect chimeric protein with low immunogenicity, its preparation method and application. The amino acid sequence of the chimeric protein is as shown in SEQ ID NO.11 or SEQ ID NO.12. The chimeric protein of the invention makes staphylokinase delta Sak with 10 amino acids deleted on the N end and staphylococcal enterotoxins delta SEC2 with 10 and 132 amino acids deleted respectively on the N and the C end jogged together, and keeps no connecting peptide between the two. The constructed chimeric proteins delta SEC2-delta Sak and delta Sak-delta SEC2 have substantially reduced molecular weights, and amino acid sequences with high immunogenicity are removed, so that the problems of large molecular weight and high immunogenicity of chimeric proteins are solved. Meanwhile, the anti-tumor and thrombolytic functions are retained.
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Description

technical field

[0001] The invention relates to low immunogenic anti-tumor thrombolytic double-effect chimeric protein and its preparation method and application, belonging to the fields of microbiology, molecular biology and genetic engineering. Background technique

[0002] In 2009, the chairman of ASCO in the United States mentioned in the introduction of tumor progress that the first cause of death from cancer is the cancer itself and cancer recurrence, and the second cause of death is blood clots. The relationship between cancer and thrombus is very close. Paying attention to the prevention and treatment of cancer thrombus has become one of the key points of clinicians' work in the future.

[0003] In 1865, French doctor Armand Trousseau reported for the first time that gastric cancer patients were prone to venous thrombosis. Soon, Billroth discovered the existence of cancer cells in thrombus in 1878, and further proposed that canceration can cause coagulation disorders...

Claims

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