Quantum dot labeled antibody multicomponent non-competitive homogeneous immunoassay method for small-molecule organic matter

Abstract

The invention discloses a quantum dot labeled antibody multicomponent non-competitive homogeneous immunoassay method for a small-molecule organic matter, and relates to a quantum dot labeled antibody multicomponent non-competitive homogeneous immunoassay technology for the small-molecule organic matter. Fluorescent light emits high-efficiency water-soluble quantum dots with different wavelengths to label antibodies resisting different target analytes. Different quantum dot labeled antibodies are dispersed into a phosphate buffering solution, and a sample solution containing the corresponding target analytes is added into the phosphate buffering solution and uniformly mixed with the phosphate buffering solution, so that non-competitive homogeneous immunoassay reaction is performed; respective labeled samples, samples and a blank contrast system which are subjected to reaction and balanced are directly subjected to fluorescent scanning or multi-wavelength detection under the excitation of ultraviolet light with the same wavelength; and the high-sensitivity quantum dot labeled antibody multicomponent non-competitive homogeneous immunoassay technology for quickly detecting various target small-molecule organic matters is constructed according to a rule that a ratio of the characteristic fluorescent light intensity of the blank contrast system to the characteristic fluorescent light intensity of a sample system is in direct proportion to the concentration of the corresponding target analytes in a certain range.

Description

Technical field [0001] The invention relates to a quantum dot-labeled antibody multi-component non-competitive homogeneous immunoassay technique for small molecular organics (pesticides, drugs, environmental endocrine disruptors). Background technique [0002] The residues of pesticides, drugs, and environmental endocrine disruptors in the environment and agricultural and livestock products exceed the standard, posing a certain threat to the environment and environmental organisms, and affecting food safety and human health. Enhancing environmental and food safety monitoring requires efficient and rapid analysis techniques. [0003] At present, the methods for detecting multiple residues of pesticides, drugs, and environmental endocrine disruptors that have been reported mainly include chromatography and color-mass spectrometry. The use of these methods requires expensive equipment, specialized laboratories, and well-trained professionals. The sample preparation requirements are h...

AI Technical Summary

Problems solved by technology

The use of these methods requires expensive instruments and equipment, specialized laboratories, and well-trained professionals. The sample pretreatment requires high requirements, the process is complicated, the speed is slow, the detection cost is high, and the specificity is not strong. It is difficult to adapt to a large number of samples and on-site rapid Testing requirements

Existing multi-component immunoassays for small molecule organics usually use different antibodies or antigens to be immobilized on different parts of polystyrene microwell plates, and different immune reactions are carried out through spatial resolution. Realize solid-liquid separation, complex operation; or use different markers, such as enzymes, fluoresceins, etc. to label different antibodies or antigens, the reaction conditions and detection methods are difficult to be compatible

Therefore, the true multi-component immunoassay technology for small molecule organics has yet to be established.

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