Lectin chip and application thereof

A lectin and chip technology, applied in the field of prevention and treatment of Kashin-Beck disease, can solve the problems of difficult pathogenic factors, low molecular level damage, difficult early intervention measures, treatment techniques and measures, etc., and achieve the effect of accurate detection

Inactive Publication Date: 2012-06-20
XI AN JIAOTONG UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] At present, the main technical problems affecting the prevention and treatment of Kashin-Beck disease in my country include: the clinical diagnosis of Kashin-Beck disease is mainly based on the appearance of clinical symptoms and signs, such as the diagnosis of metaphyseal and bone end lesions on the right hand X-ray It is an early Kashin-Beck disease, which obviously lags behind the chondrocyte necrosis and biochemical damage of Kashin-Beck disease

Method used

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  • Lectin chip and application thereof
  • Lectin chip and application thereof
  • Lectin chip and application thereof

Examples

Experimental program
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Embodiment Construction

[0025] In order to facilitate the study of the molecular pathogenesis of Kashin-Beck disease and the prognosis monitoring after treatment, the serum samples of patients with Kashin-Beck disease after the onset of Kashin-Beck disease were analyzed by detecting the serum samples of healthy people, the serum samples of untreated Kashin-Beck patients and the serum samples of Kashin-Beck patients after hyaluronic acid injection. Changes in the sugar chain spectrum of glycoproteins in serum, looking for glycoproteins that can be used as indicators for the detection of Kashin-Beck disease. The present invention will be further described in detail below in conjunction with the detection of specific lectin chips, which is an explanation of the present invention rather than a limitation.

[0026] 1. Processing of serum samples:

[0027] 1.1 Collection of serum samples

[0028] A total of 25 serum samples from healthy people in KBD wards, 16 serum samples from patients with Kashin-Beck ...

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Abstract

The invention discloses a lectin chip and application thereof. The lectin chip comprises a solid phase carrier and lectin probes, wherein the lectin probes are fixed on the solid phase carrier and distributed in an array manner; and the lectin probes comprise a type-I lectin probe, a type-II lectin probe and a control probe. The lectin chip manufactured by the two types of probes can be applied to research on molecular pathogenesis of the Kaschin-Beck disease and prognosis monitoring after treatment on the Kaschin-Beck disease by detecting the change of the glycoprotein content identified by the lectin probes in a fluorescence labeled serum sample according to fluorescence labeled glycoprotein qualitative or quantitative judgment.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of Kashin-Beck disease, and relates to a lectin chip and application thereof. Background technique [0002] Kaschin-Beck disease (Kashin-Beck Disease) is an endemic bone and joint disease and one of the key endemic diseases in my country. According to the Kaschin-Beck disease prevention and control situation issued by the Ministry of Health in October, 2010, my country's Kaschin-Beck disease is mainly prevalent in 366 counties in 14 provinces (cities) and autonomous regions from the northeast to the Sichuan-Tibet Plateau. There are 694,500 Kashin-Beck disease patients and above, including 18,435 patients under the age of 13, and 105.838 million people are threatened. Kashin-Beck disease not only caused great physical and mental pain to the patients, but also brought heavy medical and financial burdens to the local area, which seriously hindered the local economic development, and ...

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Application Information

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IPC IPC(8): G01N33/543G01N33/68
Inventor 郭雄李铮简强于汉杰谭武红雷艳霞张峰马玮娟韩晶武世勋王森李春燕何淑兰吴翠艳宁玉洁
Owner XI AN JIAOTONG UNIV
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