Lectin chip and application thereof

Abstract

The invention discloses a lectin chip and application thereof. The lectin chip comprises a solid phase carrier and lectin probes, wherein the lectin probes are fixed on the solid phase carrier and distributed in an array manner; and the lectin probes comprise a type-I lectin probe, a type-II lectin probe and a control probe. The lectin chip manufactured by the two types of probes can be applied to research on molecular pathogenesis of the Kaschin-Beck disease and prognosis monitoring after treatment on the Kaschin-Beck disease by detecting the change of the glycoprotein content identified by the lectin probes in a fluorescence labeled serum sample according to fluorescence labeled glycoprotein qualitative or quantitative judgment.

Description

technical field [0001] The invention belongs to the technical field of prevention and treatment of Kashin-Beck disease, and relates to a lectin chip and application thereof. Background technique [0002] Kaschin-Beck disease (Kashin-Beck Disease) is an endemic bone and joint disease and one of the key endemic diseases in my country. According to the Kaschin-Beck disease prevention and control situation issued by the Ministry of Health in October, 2010, my country's Kaschin-Beck disease is mainly prevalent in 366 counties in 14 provinces (cities) and autonomous regions from the northeast to the Sichuan-Tibet Plateau. There are 694,500 Kashin-Beck disease patients and above, including 18,435 patients under the age of 13, and 105.838 million people are threatened. Kashin-Beck disease not only caused great physical and mental pain to the patients, but also brought heavy medical and financial burdens to the local area, which seriously hindered the local economic development, and ...

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Problems solved by technology

[0004] At present, the main technical problems affecting the prevention and treatment of Kashin-Beck disease in my country include: the clinical diagnosis of Kashin-Beck disease is mainly based on the appearance of clinical symptoms and signs, such as the diagnosis of metaphyseal and bone end lesions on the right hand X-ray It is an early Kashin-Beck disease, which obviously lags behind the chondrocyte necrosis and biochemical damage of Kashin-Beck disease, and even lags behind the damage at the molecular level under the long-term exposure of the population living in the ward area to harmful environmental factors. Therefore, research based on the level of clinical symptoms and signs The etiology of Kashin-Beck disease of unknown cause is difficult to find the pathogenic factors, breakthrough etiology research, and it is difficult to establish early intervention measures and treatment techniques and measures

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