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Method for screening and identifying tumor/testis antigens based on monoclonal antibodies with spermatogenic cell specificities

A monoclonal antibody and testicular antigen technology, which can be used in measurement devices, instruments, scientific instruments, etc., can solve problems such as the inability to meet tumor immunology and the inability to effectively identify differentially expressed proteins.

Inactive Publication Date: 2012-06-20
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

Therefore, the tumor / testis antigen gene expression analysis method cannot effectively identify differentially expressed proteins. For example, the MPSS method, which has attracted much attention from researchers, only identified a tumor / testis antigen in CT45
Obviously, the current identification methods of tumor / testis antigens cannot satisfy the research of tumor immunology

Method used

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  • Method for screening and identifying tumor/testis antigens based on monoclonal antibodies with spermatogenic cell specificities
  • Method for screening and identifying tumor/testis antigens based on monoclonal antibodies with spermatogenic cell specificities
  • Method for screening and identifying tumor/testis antigens based on monoclonal antibodies with spermatogenic cell specificities

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Embodiment Construction

[0028] The method for screening and identification of tumor / testis antigens provided by the present invention uses human sperm cells as immunogens, applies lymphocyte hybridoma technology to prepare monoclonal antibodies, and uses immunohistochemical method to screen human sperm cell-specific monoclonal antibodies; On the horizontal level, the expression distribution of the antigen molecules recognized by the screened monoclonal antibodies in tumor tissues and other normal tissues was investigated. Based on this, immunohistochemical staining was used to select monoclonal antibodies against the regular expression of tumor / testis antigens (that is, anti-human sperm cell-specific monoclonal antibodies that only react with human tumor tissues but not with normal human tissues), As a candidate antibody recognizing tumor / testis antigen; through immunoprecipitation-mass spectrometry and other modern proteomics techniques, screen and identify the new tumor / testis antigen molecule recog...

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Abstract

The invention discloses a method for screening and identifying tumor / testis antigens based on monoclonal antibodies with spermatogenic cell specificities. Spermatogenic cells of human bodies are used as immune antigens, monoclonal antibodies are prepared by the aid of the lymphocyte hybridoma technology, the monoclonal antibodies with spermatogenic cell specificities are screened by the aid of immunohistochemistry, and then the expression distribution law of antigens identified on the screened monoclonal antibodies on tumor tissues and other normal tissues is researched on a protein level. The monoclonal antibodies are selected for the expression law of the tumor / testis antigens by the aid of immunohistochemical staining on the basis to be used as candidate antibodies for identifying the tumor / testis antigens, and new tumor / testis antigen molecules recognized by the monoclonal antibodies can be identified by the aid of proteomics technologies such as immunoprecipitation-mass spectrum analysis and the like.

Description

technical field [0001] The invention belongs to the technical field of tumor antigen screening, and relates to a method for screening and identifying tumor / testis antigens based on germ cell-specific monoclonal antibodies. Background technique [0002] Malignant tumors are currently one of the major diseases that seriously affect human health and threaten human life. Therefore, the World Health Organization and government health departments of various countries have listed cancer as a top priority. At the 18th International Anti-Cancer Union Conference, a research report published by the World Health Organization showed that the global cancer situation will become increasingly serious, and the number of new patients will increase from the current 10 million to 15 million per year in the next 20 years. The number of people will also increase from 6 million to 10 million per year. In response to the above severe situation, the report calls on all countries to formulate nation...

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Application Information

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IPC IPC(8): G01N33/577G01N30/02
Inventor 杨琨宋朝君金伯泉
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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