Color reagent made from colorimetry nanometer material and used for detecting melamine and application of color reagent

A chromogenic reagent, melamine technology, applied in the field of analysis and detection, can solve the problems of destroying the optical properties of nanoparticles and the complicated preparation process of nanomaterials, and achieve the effect of simple measurement, simple preparation method, sensitive and selective response

Active Publication Date: 2014-07-16
合生元(长沙)营养食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using specific substances to modify nanoparticles makes the preparation process of nanomaterials complicated, and the modification process often destroys the optical properties of nanoparticles

Method used

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  • Color reagent made from colorimetry nanometer material and used for detecting melamine and application of color reagent
  • Color reagent made from colorimetry nanometer material and used for detecting melamine and application of color reagent
  • Color reagent made from colorimetry nanometer material and used for detecting melamine and application of color reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] a. There is 2.0×10 in the melt -4 Add 14.8mg of trisodium citrate to 50mL of mol / L silver nitrate aqueous solution and stir electromagnetically;

[0019] b. Add 1.0 mL of 0.2mol / L sodium borohydride solution to the above mixed solution, and react at 20°C for 2 hours to obtain water-soluble silver nanomaterials.

[0020] c. Add 1mL of the obtained water-soluble silver nanomaterial into a glass colorimetric tube, and then add a certain concentration of the melamine solution to be tested, and finally, add 1mL of 0.01mol / L hydrogen peroxide aqueous solution and dilute to 5mL with deionized water , Observe the color and record the absorption spectrum after 10 minutes of color reaction.

Embodiment 2

[0022] 1.0×10 -4 mol / L chloroauric acid and 1.0×10 -4 Add 28.8mg of trisodium citrate to 50mL of mol / L silver nitrate aqueous solution and stir electromagnetically;

[0023] b. Add 5.0mL of 0.2mol / L ascorbic acid solution to the above mixed solution, react at 50°C for 0.5 hours to obtain water-soluble gold-silver composite nanomaterials;

[0024] c. Add 0.2mL of the obtained water-soluble gold-silver nanomaterial into the glass colorimetric tube, then add a certain concentration of the melamine solution to be tested, and finally, add 1mL 1.0×10 -4 The mol / L ozone aqueous solution is diluted to 5 mL with deionized water, the color is observed after 5 minutes of color reaction and the absorption spectrum is recorded.

Embodiment 3

[0026] a. 1.0×10 in melt -4 mol / L copper sulfate and 2.0×10 -4 Add 47.4mg of trisodium citrate to 50mL of mol / L silver nitrate aqueous solution and stir electromagnetically;

[0027] b. Add 0.1 mL of 0.2 mol / L hydrazine hydrate solution to the above mixed solution, and react at 30° C. for 1 hour to obtain water-soluble copper-silver nanomaterials.

[0028] c. Add 0.2mL of the obtained water-soluble copper-silver nanomaterial into a glass colorimetric tube, then add a certain concentration of the melamine solution to be tested, and finally, add 2mL 1.0×10 -2 The mol / L ferric nitrate aqueous solution is diluted to 5 mL with deionized water, the color is observed after 20 minutes of color reaction and the absorption spectrum is recorded.

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Abstract

The invention provides a color reagent made from colorimetry nanometer material and used for high sensitively and high selectively detecting melamine and application of the color reagent. A main body of the color reagent is formed by a metal nanometer material which is in single or composite composition, and the obtained metal nanometer material can be used for carrying out a color reaction with a melamine solution under the condition that a corrosive reagent of the metal nanometer material exists. A water-soluble metal nanometer optical probe manufactured by the color reagent disclosed by the invention has a stronger plasma resonance absorption effect in a visible region, the optical probe which is used as the color reagent has a highly-sensitive selectivity response for the melamine, the lowest concentration which can be detected by a visual method can be 0.6nM, and the visual method is the method with the lowest detection limit in the colorimetry reported at present. Compared with the method for detecting the melamine with the metal nanometer material reported at present, the method provided by the invention has the advantages that: the nanometer material does not need to be specially modified, and thus the method is more simple and convenient. The method provided by the invention is hopeful to be used for the simple, convenient and efficient detection for the melamine in food comprising milk products in liquid state or solid state.

Description

Technical field: [0001] The invention relates to the field of analysis and detection, in particular to the application of water-soluble, high-absorption coefficient metal nanomaterials as colorimetric reagents in rapid detection of melamine. Background technique: [0002] Melamine (2,4,6-triamino-1,3,5-triazine, MA) is a triazine nitrogen-containing heterocyclic compound, which is widely used in polymer resins and organic chemical raw materials. Since melamine has a high nitrogen content (about 66%) and its price is relatively cheap, some criminals add it to a variety of foods including dairy products and livestock and poultry feeds and other human or animal foods to manufacture The illusion of high protein content [JHYu, CC Zhang, P. Dai, SGGe, Anal. Chim. Acta 651 (2009) 209-214; JB Wang, N. Moussa, K. Hisahiro, M. Koji, K. Fujio, Food Chem. Toxicol. 48 (2010) 1791-1795]. The reason is that the Kjeldahl method and the Dumas method are commonly used in food testing to detect p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 王光丽朱晓瑛董玉明张帝漆马茜
Owner 合生元(长沙)营养食品有限公司
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