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Quality standard of edible gastrodia elata

A quality standard and technology of Gastrodia elata, applied in the field of quality standards for edible Gastrodia elata, can solve the problems of Gastrodia elata quality standard blank, Gastrodia elata is not a dual-purpose variety for medicine and food, etc.

Inactive Publication Date: 2012-08-15
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to the relevant regulations of the country, Gastrodia elata is not a dual-purpose variety for medicine and food at present.
The quality standard of edible gastrodia elata is still blank

Method used

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  • Quality standard of edible gastrodia elata

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0005] Quality determination of edible Gastrodia elata by high performance liquid chromatography

[0006] (1) Instruments and reagents: Shimadzu LC-20AT high performance liquid chromatograph (Shimadzu, Japan), chromatographic column: Thermo C18 column (250mm×4.6mm, 5μm) Shumei KQ-3200E ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), Sartorius electronic balance (Sartorius, Germany), methanol is chromatographic grade (Fisher Company), and high-purity water (Hangzhou Wahaha Co., Ltd.).

[0007] (2) Selection of experimental conditions:

[0008] Extraction method: Ultrasonic extraction with 50% methanol for 40 minutes, methodological investigation: the extraction rate of this method is high, accurate and feasible.

[0009] Detection wave: 224nm.

[0010] Chromatographic conditions: methanol-water=26:74 as mobile phase, column temperature 40°C. Under the chromatographic conditions, the retention time of the reference substance is 16.1min, and the number of theoret...

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Abstract

The invention relates to a content measurement method of parishin in edible gastrodia elata, characterized in that a high performance liquid chromatographyparishin method is used for the mass measurement, a high performance liquid chromatograph is used, a chromatographic column is a C18 column, an extraction method comprises: ultrasonically extracting for 40min by using 50% of methyl alcohol; the detection wavelength is 224nm; the chromatography condition comprises: a moving phase comprises the methyl alcohol and water with a ratio of 26:74 and the column temperature is 40 DEG C; a contrast solution and a test solution are accurately and respectively sucked for 5 mul and injected into the liquid chromatograph for measurement.

Description

technical field [0001] The invention relates to a quality standard for edible gastrodia elata. Background technique [0002] Gastrodia elata is the dry tuber of Gastrodia elata B1., also known as Chijian, Mokpo, or Astragalus. It is mainly produced in Guizhou, Sichuan, Yunnan, Hubei, Shaanxi and other places. Sweet in nature, flat to the liver meridian, has the effects of calming the liver, calming wind, and relieving spasm. It is clinically used for headache, dizziness, limb numbness, convulsions in children, epileptic convulsions, and tetanus. As a medicine, the 2010 edition of the Chinese Pharmacopoeia stipulates the use of gastrodin as a test index for the medicinal material of Gastrodia elata. In addition, there are other reports on the content determination method of Gastrodia elata as a drug. [0003] Traditionally, Gastrodia elata has the habit of eating. However, according to the relevant regulations of the country, Gastrodia elata is not a dual-purpose variety ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/90G01N21/33
Inventor 刘安章军
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI