Device for classifying and counting white cells by using even high-order aspherical laser shaping system

A high-order aspheric surface and white blood cell classification technology, which is applied in the field of white blood cell differential counters, can solve problems such as white blood cell count errors and unclear classification boundaries, and achieve the goal of reducing misjudgment, reducing unclear classification boundaries, and reducing scattered light intensity deviations Effect

Active Publication Date: 2012-09-19
DIRUI MEDICAL TECH CO LTD
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Problems solved by technology

When the scattered light of different sizes is collected and processed, it will be misjudged as different samples, and a large sample may be misjudged as a small sample, which will make the one-...

Method used

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  • Device for classifying and counting white cells by using even high-order aspherical laser shaping system
  • Device for classifying and counting white cells by using even high-order aspherical laser shaping system
  • Device for classifying and counting white cells by using even high-order aspherical laser shaping system

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Embodiment Construction

[0044] Including laser shaping illumination unit, sample processing delivery unit and subsequent signal processing unit, wherein,

[0045] The laser shaping lighting unit includes a laser and a laser shaping module, and the laser shaping module performs collimation, uniformity, and convergence shaping on the laser light emitted by the laser;

[0046] The sample processing and delivery unit includes an air pressure transmission control module and a liquid transmission control module, and is connected to the sheath flow cell, so that the sample fluid containing the measured white blood cells is carried by the sheath fluid and flows through the sheath flow cell;

[0047] The light spot generated by the laser shaping lighting unit is irradiated on the sample liquid passing through the sheath flow cell;

[0048] The subsequent signal processing unit includes an optical signal processing module, a photoelectric conversion module, and an electrical signal processing module. The subse...

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Abstract

The invention relates to a device for classifying and counting white cells by using an even high-order aspherical laser shaping system and belongs to optics. The device comprises a laser shaping and lighting unit, a sample processing and transmission unit and a subsequent signal processing unit, wherein a laser shaping module at least comprises a uniformizing and collimating lens which is used for collimating and uniformizing the Gauss beam emitted by a laser; the sample processing and transmission unit comprises an air pressure transmission control module, a liquid transmission control module and a sheath flow pool; and the subsequent signal processing unit comprises an optical signal processing module, a photovoltaic conversion module and an electrical signal processing module. The device has the advantages that when the Gauss beam emitted by the laser is collimated, the light energy density which is distributed on the cross section of a light beam in a Gauss shape is adjusted to be distributed nearly flatly, so that the scattering light intensity is slightly deviated, and the problems of erroneous judgment and unclear classification limits during subsequent signal processing are solved.

Description

technical field [0001] The present invention relates to optics, in particular to a white blood cell differential counter. Background technique [0002] Traditional flow cytometry has been widely used in the field of leukocyte differential counting. Such a white blood cell differential counter is mainly composed of an illumination unit, a sheath flow cell unit, a pressure fluid circuit unit and a signal processing unit. Among them, the illumination unit collimates and shapes the light emitted by the light source (usually a laser) into an elliptical light spot in cross section, and irradiates it to the sheath flow pool. The sheath flow cell provides an optical detection area in which the leukocyte sample is encased in the sheath flow using the principle of sheath flow, allowing the leukocytes to pass through the area one by one. After the cells passing through this area are illuminated by the lighting unit, they emit scattered light in the whole space; the signal processing ...

Claims

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Application Information

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IPC IPC(8): G01N15/14
Inventor 宋洁朱海波丁立明韩闯
Owner DIRUI MEDICAL TECH CO LTD
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