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Establishing method of symbiont of cyclobalanopsis glaucoides and bolete

A method of establishment, the technology of Qinggang yunnanensis, applied in the cultivation field of pure symbiotic relationship between Qinggang yunnanensis and boletus, can solve the problems that the interference of other microorganisms cannot be ruled out, and the types of root microorganisms are complicated, etc., and achieve the effect of easy operation and simple preparation

Inactive Publication Date: 2014-04-02
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when studying the symbiosis of boletus and plants, the types of root microorganisms in the natural environment are complex, and the interference of other microorganisms on plant roots cannot be ruled out, which limits the application of a number of modern technologies in the study of the symbiosis mechanism of boletus and plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0017] The seeds of Dianqinggang were collected in the broad-leaved forest of Lion Mountain in Wuding, Yunnan; the seeds were put into clean water, the sediment and dirt were washed away, and the rotten seeds floating on the water surface were removed; the selected seeds were naturally dried, and the seed coats were removed. Select the seeds without obvious plaque in the cotyledons; place the selected seeds in 75% ethanol solution, shake and soak for 30 s, take out the seeds, and wash them twice with sterile water; place the seeds in 0.1% HgCl 2 solution, shake and soak for 10 min, take out the seeds, and wash them with sterile water for 2 to 3 times; put the sterilized seeds with germs facing up into the seed germination medium (MS medium, sugar-free, 6-BA 1.50mg / L, NAA 0.10mg / L, agar 7.50 g / L, pH 6.80), inoculated 1-2 seeds per bottle, uncontaminated brown seeds began to germinate after dark culture at 21-22°C for 10 days, and transferred to 2000 Cultivate under the light o...

example 2

[0021] The seeds of Dianqinggang were collected in the broad-leaved forest of Lion Mountain in Wuding, Yunnan; the seeds were put into clean water, the sediment and dirt were washed away, and the rotten seeds floating on the water surface were removed; the selected seeds were naturally dried, and the seed coats were removed. Select the seeds without obvious plaque in the cotyledons; place the selected seeds in 75% ethanol solution, shake and soak for 30 s, take out the seeds, and wash them twice with sterile water; place the seeds in 0.1% HgCl 2 solution, shake and soak for 10 min, take out the seeds, and wash them with sterile water for 2 to 3 times; put the sterilized seeds germs upward into the seed germination medium (MS medium, sugar-free, 6-BA 1.30mg / L, NAA 0.10mg / L, agar 7.50 g / L, pH 6.80), inoculated 1-2 seeds per bottle, uncontaminated brown seeds began to germinate after dark culture at 21-22°C for 11 days, and transferred to 2000 Cultivate under the light of ~2500L...

example 3

[0025] The seeds of Dianqinggang were collected in the broad-leaved forest of Lion Mountain in Wuding, Yunnan; the seeds were put into clean water, the sediment and dirt were washed away, and the rotten seeds floating on the water surface were removed; the selected seeds were naturally dried, and the seed coats were removed. Select the seeds without obvious plaque in the cotyledons; place the selected seeds in 75% ethanol solution, shake and soak for 30 s, take out the seeds, and wash them twice with sterile water; place the seeds in 0.1% HgCl 2 solution, shake and soak for 10 min, take out the seeds, and wash them with sterile water for 2 to 3 times; put the sterilized seeds with the embryo facing up into the seed germination medium (MS medium, sugar-free, 6-BA 1.00mg / L, NAA 0.08mg / L, agar 7.50 g / L, pH 6.80), inoculated 1-2 seeds per bottle, uncontaminated brown seeds began to germinate after 9 days of dark culture at 21-22°C, and transferred to 2000 Cultivate under the ligh...

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Abstract

The invention relates to an establishing method of symbiont of cyclobalanopsis glaucoides and bolete, belonging to the field of biotechnology (plant tissues and fungal culture). In the research of mycorrhizal fungi, a stable pure symbiont of plants and fungus is difficult to establish. At present, bolete can not be cultivated artificially easily, belongs to mycorrhizal fungi capable of realizing mutualistic symbiosis with the plant, and has symbiotic relationship with pinaceae plants, fagaceae plants and the like under natural condition. According to the establishing method, cyclobalanopsis glaucoides seed and generalized delicious beef liver mushroom entity collected in the Wuding lion rock are used as materials, so that callus and mycelium are respectively induced successfully and are cultured together in an innovation manner, and a simple and stable pure-symbiont system is established under the sterile state. Important basis is provided for the symbiotic relationship and the symbiotic mechanism of bolete, cyclobalanopsis glaucoides and the other plants and the molecular mechanism of the beef liver mushroom entity and the like, and the method use for reference is provided for research on other macro fungi and symbiotic plants.

Description

technical field [0001] The invention relates to a cultivation method for establishing a pure symbiotic relationship between Qinggang yunnanensis and boletus, which belongs to the field of biotechnology, and specifically belongs to the category of plant tissue culture and large fungus mycelium culture. Background technique [0002] In the study of mycorrhizal fungi, the symbiotic relationship between plants and fungi is a hot and difficult research point. Many scholars have tried to establish a stable pure symbiont to prevent fungal infection on the premise of excluding other bacteria and environmental interference. However, there are almost no symbionts that meet the above conditions in the natural environment. [0003] Boletus ( Boletus ) belongs to Basidiomycota (Basidiomycota), Agaricomycetes (Agaricomycetes), Boletales (Boletales), Boletaceae (Boletaceae), and is a large genus widely distributed in the world. Boletus fruiting bodies are large in size, fleshy and delici...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G1/04
Inventor 李宗菊王鹏飞张曦予周文李彪吴鹏
Owner YUNNAN UNIV
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