Production process of composite probiotics

Abstract

The invention discloses a production process of composite probiotics. The process comprises the following specific steps of: 1, breeding, namely (1) preparing a culture solution, and heating, dissolving and saccharifying the culture solution to obtain a saccharified solution; (2) sterilizing the saccharified solution at a high temperature, and cooling the sterilized saccharified solution to obtain a breeding culture medium; and (3) inoculating strains of lactic acid bacteria, bifidobacterium, bacillus subtilis and candida utilis into the breeding culture medium for culturing, thus obtaining four mature bacterium solutions; 2, fermentation, namely (1) mixing and sterilizing bean pulp and bran, thus obtaining a solid material; (2) respectively adding the four mature bacterium solutions into a diluent, thus obtaining four diluted bacterium solutions after diluting; and (3) respectively inoculating strains of the four diluted bacterium solutions into the solid material for fermentation, thus obtaining four fermented materials; and 3, drying and packaging, namely (1) mixing the four fermented materials and drying the mixed materials for the first time; (2) drying the mixed materials for the second time; (3) drying the mixed materials for the third time; and (4) crushing, outputting and packaging the dried materials to obtain the composite probiotics. The process can realize large-scale and continuous production and is suitable for industrial production.

Description

Technical field

[0001] The invention belongs to the field of biotechnology, and particularly relates to a production process of composite probiotics. Background technique

[0002] With the rapid development of the current feed industry, various feed additives are also showing a diversified trend. Due to the gradual emergence of the various disadvantages of antibiotics, compound probiotics can adjust the structure of animal intestinal flora, improve the activity of digestive enzymes in animals, and improve Animal production performance and the excellent characteristics of improving animal immunity have gradually become indispensable feed additives in the animal husbandry industry.

[0003] At present, the research, development and application of composite probiotics in my country is still in its infancy. The production methods of most composite probiotics are simply the expansion of laboratory preparation methods, which are not suitable for large-scale and continuous industrial prod...

Examples

Embodiment 1

[0057] In Example 1, the specific process steps are as follows:

[0058] (1) Breeding:

[0059] ①Add 700g of barley germ, 600g of peptone, 1000g of agar, 300g of sodium citrate, 150g of potassium dihydrogen phosphate, 7.5g of magnesium sulfate, and 200g of sodium bicarbonate into the batching tank, make up to 100L of distilled water, and adjust the pH after heating to boiling Transfer to the saccharification tank after 4.5, add 2.1×10 to the saccharification tank 5 U saccharification enzyme, heat preservation and saccharification at 60°C for 2h to obtain 104.6kg saccharification liquid

[0060] ②Transfer 104.6kg of saccharification liquid into the fluid heater, heat up to 95℃ within 5s, keep it for 1h, and sterilize at high temperature. The sterilized saccharification liquid flows through the cooling pipe, and the temperature is reduced to 30℃ within 5s to obtain 103.8kg breeding Culture medium

[0061] ③Equally distribute 103.9kg of breeding medium to four chemostats, and use arrow...

Embodiment 2

[0071] In Example 2, the specific process steps are as follows:

[0072] (1) Breeding:

[0073] ①Add 2.4kg of barley germ, 1.6kg of peptone, 2.4kg of agar, 0.8kg of sodium citrate, 0.36kg of potassium dihydrogen phosphate, 0.019kg of magnesium sulfate, and 0.6kg of sodium bicarbonate into the batching tank, make up to 200L of distilled water, and heat to After boiling, adjust the pH to 5.0 and then transfer to the saccharification tank, and add 9.6×10 to the saccharification tank 5 U saccharification enzyme was incubated at 60°C for 4 hours to obtain 212.44kg saccharification liquid;

[0074] ②Transfer 212.44kg of saccharification liquid into the fluid heater, heat up to 100℃ within 8s, keep it for 1.5h, and sterilize at high temperature. The sterilized saccharification liquid flows through the cooling pipe and is cooled to 40℃ within 8s to obtain 210.18kg Breeding medium

[0075] ③Distribute 210.18kg of breeding medium evenly into four chemostats, and use arrow inoculation machine ...

Embodiment 3

[0085] In Example 3, the specific process steps are as follows:

[0086] (1) Breeding:

[0087] ①Put 2.85kg barley germ, 2.1kg peptone, 3.3kg agar, 1.05kg sodium citrate, 0.495kg potassium dihydrogen phosphate, 0.0255kg magnesium sulfate, 0.75kg sodium bicarbonate into the batching tank, make up to 300L with distilled water, and heat to After boiling, adjust the pH to 4.8 and then transfer to the saccharification tank, add 9.98×10 to the saccharification tank 5 U saccharification enzyme was incubated at 60°C for 3h to obtain 311.9kg saccharification liquid;

[0088] ②Transfer 311.9kg of saccharification liquid into the fluid heater, heat up to 98°C within 6s, keep the temperature for 1.3h, and sterilize at high temperature. The sterilized saccharification liquid flows through the cooling pipe and is cooled to 35°C within 7s to obtain 310.08kg. Breeding medium

[0089] ③Equally distribute 310.08kg of breeding medium to four chemostats, and use arrow-type inoculation machine for the s...

Technical field

[0001] The invention belongs to the field of biotechnology, and particularly relates to a production process of composite probiotics. Background technique

[0002] With the rapid development of the current feed industry, various feed additives are also showing a diversified trend. Due to the gradual emergence of the various disadvantages of antibiotics, compound probiotics can adjust the structure of animal intestinal flora, improve the activity of digestive enzymes in animals, and improve Animal production performance and the excellent characteristics of improving animal immunity have gradually become indispensable feed additives in the animal husbandry industry.

[0003] At present, the research, development and application of composite probiotics in my country is still in its infancy. The production methods of most composite probiotics are simply the expansion of laboratory preparation methods, which are not suitable for large-scale and continuous industrial prod...

Examples

Embodiment 1

[0057] In Example 1, the specific process steps are as follows:

[0058] (1) Breeding:

[0059] ①Add 700g of barley germ, 600g of peptone, 1000g of agar, 300g of sodium citrate, 150g of potassium dihydrogen phosphate, 7.5g of magnesium sulfate, and 200g of sodium bicarbonate into the batching tank, make up to 100L of distilled water, and adjust the pH after heating to boiling Transfer to the saccharification tank after 4.5, add 2.1×10 to the saccharification tank 5 U saccharification enzyme, heat preservation and saccharification at 60°C for 2h to obtain 104.6kg saccharification liquid

[0060] ②Transfer 104.6kg of saccharification liquid into the fluid heater, heat up to 95℃ within 5s, keep it for 1h, and sterilize at high temperature. The sterilized saccharification liquid flows through the cooling pipe, and the temperature is reduced to 30℃ within 5s to obtain 103.8kg breeding Culture medium

[0061] ③Equally distribute 103.9kg of breeding medium to four chemostats, and use arrow...

Embodiment 2

[0071] In Example 2, the specific process steps are as follows:

[0072] (1) Breeding:

[0073] ①Add 2.4kg of barley germ, 1.6kg of peptone, 2.4kg of agar, 0.8kg of sodium citrate, 0.36kg of potassium dihydrogen phosphate, 0.019kg of magnesium sulfate, and 0.6kg of sodium bicarbonate into the batching tank, make up to 200L of distilled water, and heat to After boiling, adjust the pH to 5.0 and then transfer to the saccharification tank, and add 9.6×10 to the saccharification tank 5 U saccharification enzyme was incubated at 60°C for 4 hours to obtain 212.44kg saccharification liquid;

[0074] ②Transfer 212.44kg of saccharification liquid into the fluid heater, heat up to 100℃ within 8s, keep it for 1.5h, and sterilize at high temperature. The sterilized saccharification liquid flows through the cooling pipe and is cooled to 40℃ within 8s to obtain 210.18kg Breeding medium

[0075] ③Distribute 210.18kg of breeding medium evenly into four chemostats, and use arrow inoculation machine ...

Embodiment 3

[0085] In Example 3, the specific process steps are as follows:

[0086] (1) Breeding:

[0087] ①Put 2.85kg barley germ, 2.1kg peptone, 3.3kg agar, 1.05kg sodium citrate, 0.495kg potassium dihydrogen phosphate, 0.0255kg magnesium sulfate, 0.75kg sodium bicarbonate into the batching tank, make up to 300L with distilled water, and heat to After boiling, adjust the pH to 4.8 and then transfer to the saccharification tank, add 9.98×10 to the saccharification tank 5 U saccharification enzyme was incubated at 60°C for 3h to obtain 311.9kg saccharification liquid;

[0088] ②Transfer 311.9kg of saccharification liquid into the fluid heater, heat up to 98°C within 6s, keep the temperature for 1.3h, and sterilize at high temperature. The sterilized saccharification liquid flows through the cooling pipe and is cooled to 35°C within 7s to obtain 310.08kg. Breeding medium

[0089] ③Equally distribute 310.08kg of breeding medium to four chemostats, and use arrow-type inoculation machine for the s...