Streptococcus mutans infection and cariogenic animal model establishing method

Abstract

The invention discloses a streptococcus mutans infection and cariogenic animal model establishing method, belonging to the technical field of microbiology. Through streptococcus mutans oral infection, a specimen is collected after 80 days, the streptococcus mutans infection is identified by adopting a plate cultivation method, and cariogenicity is observed through dyeing and teeth are evaluated. According to the invention, streptococcus mutans and Carbomer 940 are mixed, and are used for orally infecting an SD (Sprague Dawley) rat, the infection condition is easily controlled, the infection rate is high (more than 90 percent), and the infection is stable. The SD rat is infected by using the method and is fed with high-sugar feeds, and thus remarkable cariogenicity can be caused.

Description

technical field [0001] The invention relates to the technical field of medicine and biology, in particular to a method for establishing an animal model of Streptococcus mutans infection and caries. Background technique [0002] Dental caries is one of the most common human diseases, with a high incidence rate. WHO ranks it as one of the three major diseases of human beings, along with cancer and cardiovascular diseases. In the latest "Global Oral Health Report" released by the World Health Organization, it is estimated that 5 billion of the world's 6.3 billion people have suffered from dental caries. In developed countries, 60% to 90% of school-age children and most adults have suffered from dental caries. The incidence rate is 50%, and 60-90% of school-age children and nearly 100% of adults in the world have dental caries. It has become a global problem that threatens human oral health. [0003] Since the 1980s, countries have invested a lot of money in caries research. T...

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Problems solved by technology

However, this method requires complex operations, and the removal of the submandibular and sublingual glands and the ligation of the parotid ducts have greatly changed the oral physiology, so it is not suitable for cariogenic research in the normal rat oral environment and to simulate the cariogenic process of the human body

[0005] Although there have been many reports on animal models of dental caries, there are still big problems in previous animal models

First of all, the colonization of bacterial infection is unstable, and the colonization amount is also uniform. With the extension of time, the bacteria are easy to clear naturally, and the caries are not obvious, which is obviously difficult to meet the requirements of the experiment.

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