Streptococcus mutans infection and cariogenic animal model establishing method

A technology for establishing methods and animal models, applied in the field of medicine and biology, can solve problems such as difficult to achieve, large changes in oral physiology, and complexity, and achieve the effects of easy control of infection conditions, high infection rate, and stable infection.

Active Publication Date: 2015-03-04
CHENGDU OLYMVAX BIOPHARM +1
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Problems solved by technology

However, this method requires complex operations, and the removal of the submandibular and sublingual glands and the ligation of the parotid ducts have greatly changed the oral physiology, so it is not suitable for cariogenic research in the normal rat oral environment and to simulate the cariogenic process of the human body
[0005] Although there have been many reports on animal models of dental caries, there are still big problems in previous animal models
First of all, the colonization of bacterial infection is unstable, and the colonization amount is also uniform. With the extension of time, the bacteria are easy to clear naturally, and the caries are not obvious, which is obviously difficult to meet the requirements of the experiment.

Method used

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  • Streptococcus mutans infection and cariogenic animal model establishing method
  • Streptococcus mutans infection and cariogenic animal model establishing method
  • Streptococcus mutans infection and cariogenic animal model establishing method

Examples

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Embodiment 1

[0026] Example 1. Establishment of S.mutans infection and cariogenic animal model

[0027] (1) Preparation of carbomer solution

[0028] 0.4% Carbomer 940 solution: Weigh 0.4g of Carbomer 940, add 0.9% NaCl 90ml, 1M sodium hydroxide to adjust the pH to 7.0, add 0.9% NaCl to make up to 100ml, mix well, sterilize at high temperature and autoclave, and cool it for later use use.

[0029] (2) Preparation of animal infection bacterial solution

[0030] S.mutans culture: take S.mutans UA159 (purchased from American Type Culture Collection, Streptococcus mutans 700610 TM ), BHI plate (Brain Heart infusion, BDBacto TM , LOT: 7128153) resuscitation, 37°C microaerobic (85%N 2 , 8%CO 2 , 5%O 2 ) cultured for 48h. Pick a single colony and inoculate 5ml of BHI medium, 37°C microaerophilic 250rpm shaking culture for 24h, and 1:100 inoculation expansion culture for 6-7h. Adjust the bacterial concentration to 2 × 10 10 CFU / mL.

[0031] Preparation of animal infection bacterial liq...

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Abstract

The invention discloses a streptococcus mutans infection and cariogenic animal model establishing method, belonging to the technical field of microbiology. Through streptococcus mutans oral infection, a specimen is collected after 80 days, the streptococcus mutans infection is identified by adopting a plate cultivation method, and cariogenicity is observed through dyeing and teeth are evaluated. According to the invention, streptococcus mutans and Carbomer 940 are mixed, and are used for orally infecting an SD (Sprague Dawley) rat, the infection condition is easily controlled, the infection rate is high (more than 90 percent), and the infection is stable. The SD rat is infected by using the method and is fed with high-sugar feeds, and thus remarkable cariogenicity can be caused.

Description

technical field [0001] The invention relates to the technical field of medicine and biology, in particular to a method for establishing an animal model of Streptococcus mutans infection and caries. Background technique [0002] Dental caries is one of the most common diseases in human beings with a high incidence rate. WHO ranks it as one of the three major human diseases for prevention and treatment along with cancer and cardiovascular diseases. In the latest "Global Oral Health Report" released by the World Health Organization, it is estimated that 5 billion people in the world's 6.3 billion people have suffered from dental caries. In developed countries, 60% to 90% of school-age children and most adults have suffered from dental caries. The incidence rate is 50%, and 60-90% of school-age children and nearly 100% of adults in the world have dental caries. It has become a global problem that threatens human oral health. [0003] Since the 1980s, countries have invested a l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/027A61K35/744A61K47/34
Inventor 刘开云邹全明郭刚张卫军孙红武付强付启欢程子洋樊绍文卢陆
Owner CHENGDU OLYMVAX BIOPHARM
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