Method of using organic acid secretion characteristics to detect phosphorus deficiency stress resistance ability of plants

A technology of feature detection and root secretion, which is applied in the direction of measuring devices, material separation, and material analysis, can solve the problems of many parameters, great influence from the external environment, and high instrument cost, and achieve strong plant adaptability, short experiment period, and good quality. The effect of controllability

Inactive Publication Date: 2013-06-19
JIANGSU UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

This method is the most widely used detection method of adversity physiology and stress resistance, but the cost of the instrument is high, the parameters are many and easy to be confused, it is greatly affected by the external environment, and it cannot reflect the long-term changes in the plant.
[0011] There are various shortcomings in the above-mentioned methods, which are not conducive to promotion and popularization in practical applications.

Method used

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  • Method of using organic acid secretion characteristics to detect phosphorus deficiency stress resistance ability of plants
  • Method of using organic acid secretion characteristics to detect phosphorus deficiency stress resistance ability of plants
  • Method of using organic acid secretion characteristics to detect phosphorus deficiency stress resistance ability of plants

Examples

Experimental program
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Effect test

Embodiment 1

[0043] The mulberry seedlings were placed in a tray with a bottom, cultured with normal formula Hoagland (Hoagland) nutrient solution for 30 days, and then randomly divided into groups. 12 plants in each group. Different phosphorus treatments were set up, one group was cultured with normal formula (control group, normal phosphorus concentration in Hoagland’s nutrient solution), and the other group was cultured with phosphorus deficiency adversity stress treatment (phosphorus deficiency group, Hoagland’s nutrient solution). No phosphorus in the nutrient solution).

[0044] After culturing for 30 days, the root exudates of two groups of mulberry trees were collected at the same time. Add 200 mL, 1 mmol / L CaCl 2 The solution is collected in the bottom of the hole tray for 4 hours from 10:00 am to 14:00 pm. The solution in the hole plate is collected and the filtrate obtained by washing the roots of the plants with deionized water is the root exudation of the mulberry tree. Sa...

Embodiment 2

[0052] The mulberry seedlings were placed in a tray with a bottom, cultured with Hoagland nutrient solution of normal formula for 30 days, and then randomly divided into groups. 12 plants in each group. Different phosphorus treatments were set up, one group was cultured with normal formula (control group, normal phosphorus concentration in Hoagland’s nutrient solution), and the other group was cultured with phosphorus deficiency adversity stress treatment (phosphorus deficiency group, Hoagland’s nutrient solution). No phosphorus in the nutrient solution).

[0053] After culturing for 30 days, the root exudates of two groups of mulberry trees were collected at the same time. Add 200 mL, 1 mmol / L CaCl 2 The solution is collected in the bottom of the plug tray for 4 hours from 10:00 am to 14:00 pm, the solution in the plug tray is collected and the filtrate obtained by washing the roots of the plants with deionized water is the root exudation of the mulberry tree and stored at ...

Embodiment 3

[0061] The Zhugecai seedlings were placed in a hole tray with a bottom, cultured with a normal formula of Hoagland nutrient solution for 30 days, and then randomly divided into groups. 12 plants in each group. Different phosphorus treatments were set up, one group was cultured with normal formula (control group, normal phosphorus concentration in Hoagland’s nutrient solution), and the other group was cultured with phosphorus deficiency adversity stress treatment (phosphorus deficiency group, Hoagland’s nutrient solution). No phosphorus in the nutrient solution).

[0062] After culturing for 30 days, the root exudates of the two groups of Zhuge Cai were collected at the same time. Add 200 mL, 1 mmol / L CaCl 2 The solution is placed in the bottom of the plug tray, collected for 4 hours from 10:00 am to 14:00 pm, the solution in the plug tray is collected and the filtrate obtained by rinsing the roots of the plants with deionized water is the root exudate of Zhuge vegetable. Sa...

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Abstract

The invention discloses a method of using organic acid secretion characteristics to detect phosphorus deficiency stress resistance ability of plants. Detected organic acid standard solution is added into air drying rhizosphere soil samples to establish a relationship model between the concentration of the organic acid standard solution and the effective phosphorus content; a phosphorus extraction ability of the organic acid secreted by a root system on the rhizosphere soil is calculated according to the relational model; an increasing proportion between a phosphorus extraction ability of organic acid secreted by a plant root system cultured in the nutrient solution prepared in a phosphorus deficiency formula on the rhizosphere soil and the phosphorus extraction ability of organic acid secreted by a plant root system cultured in the nutrient solution prepared in a normal formula on the rhizosphere soil is calculated. Then extraction cost of the organic acids secreted by the root system on the phosphorus is calculated, and the size of the phosphorus deficiency stress resistance ability of different plants is judged. The method can quantitatively detect the phosphorus deficiency stress resistance ability of the plants, and detected results of the different plants are comparable; the method is not influenced by any time and space geographical factors, and can dynamically monitor physiological change characteristics in plants for a long term.

Description

technical field [0001] The invention relates to a method for detecting the ability of plants to resist phosphorus deficiency stress by using the secretion characteristics of root system organic acids, and belongs to the field of agricultural engineering technology and the monitoring field of drought resistance ability of crops. Background technique [0002] Phosphorus is one of the important factors limiting plant growth and development. Phosphorus deficiency seriously affects the growth and development of plants. Many studies have shown that phosphorus deficiency stress will increase the secretion of organic acids in plant roots. To activate insoluble phosphorus compounds in the soil such as calcium phosphorus, iron phosphorus and aluminum phosphorus, so as to alleviate phosphorus stress. Therefore, the increase of the content of organic acids secreted by plant roots under phosphorus deficiency stress is a response mechanism to the environmental stress. Microbial activity ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 吴沿友赵宽赵玉国邢德科
Owner JIANGSU UNIV
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