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Application of Chaetin in Preparation of Drugs for Preventing and Treating Heart Disease

A technology of chaetin and heart disease, which is applied in the application field of chaetin compound and chaetin in the preparation of drugs for the prevention and treatment of heart disease, which can solve the problems of no research reports, etc. structure and maintain the normal function of cardiomyocytes

Inactive Publication Date: 2015-08-26
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the early stage, there were reports that chaetin was used to prevent and treat tumor diseases, but there was no research report on chaetin in preventing and treating heart disease.

Method used

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  • Application of Chaetin in Preparation of Drugs for Preventing and Treating Heart Disease
  • Application of Chaetin in Preparation of Drugs for Preventing and Treating Heart Disease
  • Application of Chaetin in Preparation of Drugs for Preventing and Treating Heart Disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Protective effect of chaetocin on pressure load-induced ventricular remodeling

[0049] 1. Experimental grouping and experimental methods:

[0050] SPF grade male C57BL / 6 mice (purchased from the Model Animal Institute of Nanjing University, the same below) were selected and randomly divided into four groups for 6 to 8 weeks: sham operation group (Sham), operation group (TAC), operation + solvent control group (TAC+DMSO), operation+administration group (TAC+Chaetocin), 6 to 8 rats in each group. Mice were artificially ventilated after anesthesia, the thorax was opened at the 2nd to 3rd intercostal space on the left side of the chest, the aortic arch was separated, the aortic arch (Transverse aortic constriction, TAC) was narrowed according to the uniform standard, and the thorax was closed. Except that TAC was not performed in the sham operation group, the rest of the operation procedure was exactly the same as that of the model group. The solvent control gro...

Embodiment 2

[0060] Example 2 Myocardial protection experiment of chaetocin on acute injury

[0061] 1. Experimental grouping and experimental methods:

[0062] Male C57BL / 6 mice of SPF grade were selected and randomly divided into three groups for 6 to 8 weeks: sham operation group (Sham+DMSO), myocardial infarction group (MI+DMSO), myocardial infarction+administration group (MI+chaetin Chaetocin), 18 to 20 per group. Mice were artificially ventilated after anesthesia, and the thorax was opened at the 2nd to 3rd intercostal space on the left side of the chest, and the left anterior descending coronary artery (Blocking Left Anterior Descending Coronary Artery, LAD) was ligated according to uniform standards to make an acute myocardial infarction model and the thorax was closed. In the sham operation group, except that LAD was not performed, the rest of the operation procedure was exactly the same as that of the model group. In the myocardial infarction group and the myocardial infarction ...

Embodiment 3

[0072] Example 3 Experiment of the effect of chaetokinin on primary cardiomyocytes

[0073] 1. Experimental method:

[0074] The method of culturing primary cardiomyocytes in vitro is as follows: After 1 to 3 days of clean grade SD suckling mice were killed by cervical dislocation, the apex of the heart was taken, digested and centrifuged, and placed in 15% serum DMEM medium, 37°C, 5% carbon dioxide incubator The medium was changed after 36-48 hours of cultivation. After the cardiomyocytes were pretreated with chaetokinin with increasing concentration, the cardiomyocytes were given H 2 o 2 (in vitro simulation of oxidative stress) or antimycin A (AA) and 2-deoxyglucose (2-DG) (in vitro simulation of cardiomyocyte ischemia model). After harvesting the cells, lyse the whole protein and do Western Blot experiment.

[0075] 2. Experimental results

[0076] Depend on Figure 22 , 23 The experimental results show that in H 2 o 2 Or under the stimulation of antimycin A (AA) ...

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Abstract

The invention discloses an application of chaetocin in preparation of a medicine used for preventing and treating heart diseases. Experimental research shows that chaetocin has effects of obviously reducing myocardial fibrosis degree and increasing a pressure load for inducing heart function, can prevent myocardial hypertrophy and ventricular remodelling and delay heart failure, and can inhibit myocardial cell oxidative stress caused by acute myocardial infarction, reduce ischemic infarction area, reduce myocardial cell oxidative stress, maintain normal function of myocardial cells and delay heart failure and displays a good heart disease preventing and treating effect, and no obvious adverse effect is produced in an experiment process, so that chaetocin is hopeful of being developed into a new generation safe and effective heart disease preventing and treating medicine.

Description

technical field [0001] The invention relates to chaetocin compounds, in particular to the application of chaetin in the preparation of medicines for preventing and treating heart diseases, and belongs to the field of pharmaceutical preparations. Background technique [0002] Heart disease is one of the most important diseases that threaten human life and health. Heart failure is a pathological process in which cardiac output is reduced due to decreased cardiac pump function and cannot meet the metabolic needs of body tissues. It is the end result of the development of many diseases of the cardiovascular system. In recent years, the advent of some new treatment techniques or drugs, such as: PT apparatus, thrombolysis, coronary artery bypass grafting, etc. have made great progress in the prevention and treatment of cardiovascular diseases, especially acute myocardial infarction and cerebrovascular accidents that threaten human survival year by year. Since then, it has shown a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/548A61P9/10A61P9/04A61P9/00
Inventor 徐涌翁鑫宇赵宇豪李跃华胡媛萍宋娟
Owner NANJING MEDICAL UNIV
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