Method for measuring sensibility of rice plants to bentazone and method for screening rice plants

A bentazon and sensitive technology, which is applied in the field of screening bentazon-sensitive rice plants, can solve the problems of time-consuming, labor-intensive, complicated operation and the like

Active Publication Date: 2015-06-03
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the defects of complex operation, time-consuming and labor-consuming in the existing methods for measuring the sensitivity of rice plants to bentazone, and to provide a simple and quick method for measuring the sensitivity of rice plants to bentazone. method

Method used

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  • Method for measuring sensibility of rice plants to bentazone and method for screening rice plants
  • Method for measuring sensibility of rice plants to bentazone and method for screening rice plants
  • Method for measuring sensibility of rice plants to bentazone and method for screening rice plants

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Ke 244BS is a stable bentazone-sensitive strain bred by the inventors by crossing 8077S / SE21S, that is, it is known that the rice plants of Ke 244BS are bendazone-sensitive rice plants. Bentazone-sensitive Oryzae 244BS was treated with bensulfuron-methyl at the doses shown in Table 1. The treatment period was 3 leaves and 1 heart stage. The average plant height was measured 7 days after the drug application, and water was used as a control, and quinclorac was used as a reference. The results are shown in Table 1.

[0034] Table 1

[0035] Treatment (3 leaves 1 heart stage)

[0036] The results in Table 1 showed that the plant height of bentazone-sensitive rice plants was significantly decreased after being treated with bensulfuron-methyl, and the plant height was only 47.82% of that of the control group.

Embodiment 2

[0038] Treat the 3-leaf 1-heart stage potted plants (pots used for potted plants with a length of 18 cm, a width of 12 cm, and a height of 6 cm) with different doses of bensulfuron-methyl (0-60 μg / pot) to treat the above-mentioned bentazone-sensitive rice seedlings of the rice family 244BS 7 days later. Investigate the plant height (the length from the base of the stem to the longest leaf, which is the average number of 20 plants, unit: cm), the results are shown in Table 2.

[0039] Table 2

[0040] Treatment dose (μg / pot)

[0041] Table 2 shows that bensulfuron-methyl has a more obvious effect of reducing plant height from the dose of 20 μg / pot, and the dose of 20-25 μg / pot is in the transitional stage, and the effect of reducing plant height at a dose of 30 μg / pot and later increases is no longer obvious . The amount of bensulfuron-methyl that has a stable effect is determined to be 30-60 μg / pot, equivalent to 0.9-1.8 grams per mu. In consideration of saving res...

Embodiment 3

[0043] Bensulfuron-methyl (30 μg / pot) was used to treat 1-leaf 1-heart to 5-leaf 1-heart stage seedlings of the bentazone-sensitive Oryzaceae 244BS mentioned above in a potted plant (pot length 18cm, width 12cm, height 6cm), 7 The plant height (the length from the base of the stem to the longest leaf) was inspected in Tianhou, and the results are shown in Table 3.

[0044] table 3

[0045]

[0046] As shown in Table 3, the treatment was carried out at the stage of 1 leaf, 1 heart to 5 leaves, 1 heart, and there was a significant difference in relative plant height between the treatment and the control; but the seedlings were too small (1 leaf, 1 heart to 2 leaves, 1 heart) and the plant height was different after treatment If the value is not large, it is not conducive to field selection, and if it is too small, it is not conducive to transplanting seedlings; the seedlings are treated with 5 leaves and 1 heart, and the seedling age exceeds the general requirement when the r...

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Abstract

The invention discloses a method for measuring the sensibility of rice plants to bentazone. The method comprises the following steps that (1) bensulfuron methyl is applied to rice seedlings of rice plants to be measured, and the rice seedlings continue to be planted after the bensulfuron methyl is applied; (2) the heights of the rice seedlings which are processed by the bensulfuron methyl is measured; (3) the heights of the rice seedlings which are processed by the bensulfuron methyl are compared with the heights of rice seedlings which are not applied with bensulfuron methyl and are the same with the rice seedlings which are processed by the bensulfuron methyl in seedling age, and if the heights of the rice seedlings which are processed by the bensulfuron methyl are lower than the heights of the rice seedlings which are not applied with bensulfuron methyl and are the same with the rice seedlings which are processed by the bensulfuron methyl in seedling age, it is determined that the rice seedlings of the rice plants to be measured are sensitive to bentazone. The invention further provides a method for screening rice plants which are sensitive to bentazone. By means of the methods, the sensibility of rice plants to bentazone can be simply and quickly measured, the methods can not cause death of the rice plants to be measured, and the rice plants measured through the methods still have the ability of breeding.

Description

technical field [0001] The invention relates to the field of agricultural breeding, in particular to a method for determining the sensitivity of rice plants to bendazone and a method for screening rice plants sensitive to bendazone. Background technique [0002] The two-line method hybrid rice uses the photo-temperature-sensitive genic male sterile line whose sterility is regulated by light and temperature. The high-temperature sterile male sterile line generally maintains sterility when the daily average temperature is above 24°C, and recovers when the daily average temperature is below 23°C. In order to be fertile, low-temperature sterile CMS lines generally remain sterile when the average daily temperature is below 29°C, and become fertile when the average daily temperature is above 30°C. However, the change of temperature is often beyond people's expectations. The abnormal change of temperature in the expected sterile period can cause the sterile line in the seed product...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01H1/04
Inventor 肖国樱周浩李锦江陈芬孟秋成于江辉
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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