Alternative solid-liquid culture method of Amanita hongtuo mycelium

Abstract

The invention relates to a method for alternating solid-liquid culture of Amanita amanita mycelium, belonging to the technical field of large fungus culture. It is characterized in that: the induced and multiplied mycelia are cultured alternately with solid and liquid, that is, after 4 to 6 generations of solid culture, 3 to 4 generations of liquid culture, and then solid culture, the growth rate of the mycelium is 8 to 10 times before the liquid culture, the growth was significantly accelerated. The invention speeds up the propagation speed of the mycelium by simply changing the culture mode, and the operation is simple and easy to realize, the benefit is obvious, and the production cost is low. Amanita toxins have potential applications in the development of new specific drugs such as antitumor, antibacterial and antiviral, sedative or anesthetic drugs, but so far it has been difficult to develop and apply due to bottlenecks such as rare resources and difficulty in artificial domestication. The present invention focuses on the problem of restricting pure culture such as the very slow growth of Amanita hongtuo mycelia, and provides conditions for large-scale cultivation of mycelia and artificial domestication cultivation in the future.

Description

technical field [0001] The invention relates to a method for solid-liquid alternate cultivation of Amanita amanita mycelium, which belongs to the field of biotechnology, and specifically belongs to the category of indoor cultivation of large poisonous fungi. Background technique [0002] Amanita ( Amanita ) belongs to Basidiomycotina, Phytomycetes, Agaricales, Amanitaceae ( Amanitaceae ), is a more special and valuable worldwide widespread genus among poisonous macrofungi, and its species diversity is very rich. Nearly 400 species have been reported in the world, and nearly 100 species (including subspecies, variants and variants) have been recorded in China. According to research, there are still many species in China that have not yet been studied and named. But now with the emergence of the global ecological crisis, many large fungal resources in my country are in danger, in a serious decline and endangered state, and some species are facing the risk of extinction bef...

AI Technical Summary

Problems solved by technology

[0004] At present, the main bottlenecks that lead to the difficulty in the development and sustainable utilization of Amanita are: ① Amanita resources are rare, and its population is small, with few individuals, low yield, and extremely limited distribution. In addition, it is sensitive to habitat. Once the habitat is destroyed, it is easy to Disappeared or extinct, belonging to a special group of endangered organisms, which increases the difficulty of its further research and utilization; ②Amanita, most of which belong to ectomycorrhizal fungi, most of which are still difficult to cultivate in nature Or uncultivated microorganisms, difficult to artificial, semi-artificial cultivation

Therefore, there is no toxin product that can be developed on a large scale at home and abroad, and the peptide toxins used as biochemical reagents are still expensive (about 100,000 US dollars per gram more than 10 years ago—Chen Zuohong et al., 1999), which is difficult to meet the needs of scientific research and application required

[0005] The pure culture of Amanita amanita is the premise or key to ensure the sustainable utilization of Amanita amanita resources, but it is still a problem that is difficult to overcome, and there are many blind spots. Difficulty in inducing filaments and very slow mycelial growth are important links or factors that restrict and affect pure culture