Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

New mutation sites associated with streptomycin resistance in Mycobacterium tuberculosis and their applications

A technique for Streptomycin bacillus and branch of tuberculosis, which is applied in the detection/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. It can solve problems such as time-consuming, affecting patient diagnosis and treatment, and increasing the chance of drug-resistant strains spreading. Achieve the effects of shortening the diagnosis time, saving treatment time and cost, and improving the level of molecular detection

Inactive Publication Date: 2017-07-07
广东省结核病控制中心
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods mainly use genetic rPS The A→G mutation standard of the 43rd base is used to detect whether the same mutation occurs in Mycobacterium tuberculosis in the sample, so as to quickly determine whether Mycobacterium tuberculosis is resistant to streptomycin, but there are still many streptomycin-resistant Mycobacterium tuberculosis cannot be detected, false negative
[0005] The high drug resistance rate of tuberculosis is an important reason for the difficulty of controlling the tuberculosis epidemic. However, the traditional drug susceptibility test of Mycobacterium tuberculosis takes a long time, affects the diagnosis and treatment of patients, and increases the chance of drug-resistant strains spreading. Therefore, the rapid identification of tuberculosis by molecular biological methods can be effectively improved. The detection rate and accuracy of mycobacterial resistance are of great significance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New mutation sites associated with streptomycin resistance in Mycobacterium tuberculosis and their applications
  • New mutation sites associated with streptomycin resistance in Mycobacterium tuberculosis and their applications
  • New mutation sites associated with streptomycin resistance in Mycobacterium tuberculosis and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Prediction of base mutation sites associated with STR drug resistance

[0026] First, using deep-sequencing technology, the whole genome of 161 strains of Mycobacterium tuberculosis from China was sequenced, including 44 strains of sensitive strains, 94 strains of multidrug-resistant strains (MDR), and 23 strains of extensively drug-resistant strains (XDR). The H37Rv standard Mycobacterium tuberculosis strain genome sequence was used as a control for bioinformatics analysis, and a batch of new genes closely related to drug resistance and single nucleotide polymorphism (SNP) sites in the intergenic region (IGR) were found, among which The single-base mutation site in the intergenic region Rv1194c-Rv1195 has a high correlation with streptomycin (STR) resistance, with a P value of 0.002, as shown in Table 1.

[0027] Table 1 Correlation between the single base mutation site in the intergenic region Rv1194c-Rv1195 and different drug resistance

[0028]

[0029] Table...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a new mutation site related to streptomycin resistance of Mycobacterium tuberculosis and its application. The new mutation site is located at the 1338631st position of the standard strain H37Rv genome, that is, the 47th base of the intergenic region Rv1194c‑Rv1195 , the point mutation that occurs is the mutation of base C to A. The new mutation site found in the present invention can be used as a detection target in the detection of streptomycin resistance of Mycobacterium tuberculosis, improve the molecular detection level of streptomycin resistance of Mycobacterium tuberculosis, shorten the diagnosis time of patients, save Patient treatment time and cost. The invention shows that the tuberculosis drug-resistant mutation site may also occur in the non-coding region, suggesting that the base mutation in the intergenic region may also be related to drug resistance, and there are also drug-resistant biomarkers. The invention provides a new idea for finding new and more effective tuberculosis drug resistance molecular markers.

Description

technical field [0001] The invention relates to high-throughput screening and verification of drug-resistant base mutation sites of Mycobacterium tuberculosis, in particular to the screening and verification of drug-resistant mutation sites of Mycobacterium tuberculosis streptomycin. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis infection. Mycobacterium tuberculosis may invade various organs of the human body, but mainly invades the lungs, which is called pulmonary tuberculosis. By the late 1980s, due to factors such as poverty, AIDS, population migration, and drug resistance, the incidence and mortality of tuberculosis rose rapidly around the world, becoming the most serious infectious disease in the world alongside AIDS. The drug resistance of Mycobacterium tuberculosis is serious, and the drug resistance rate is as high as 46%, which poses a severe challenge to the prevention and treatment of tuberculosis....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68C12Q1/04
Inventor 周琳钟球毕利军郭卉欣陈涛李海成陈亮陈瑜晖蒋莉舒杨王威
Owner 广东省结核病控制中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products