New mutation sites associated with isoniazid resistance in Mycobacterium tuberculosis and their application

A technology for Mycobacterium tuberculosis and drug-resistant mutations, applied in the direction of microbial-based methods, microbial measurement/inspection, biochemical equipment and methods, etc., can solve the problems of increasing the chance of spreading drug-resistant strains, taking a long time, and affecting patient diagnosis and treatment, etc. problems, to achieve the effect of improving molecular detection level, shortening diagnosis time, saving treatment time and cost
CN103820440BInactive Publication Date: 2017-07-11广东省结核病控制中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广东省结核病控制中心
Publication Date
2017-07-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a new mutation site related to isoniazide resistance of mycobacterium tuberculosis and an application thereof. The new mutation site is positioned at a 1673425th site of a standard strain H37Rv genome, that is a 126th base in an intergenic region of Rv1482c to Rv1483*, and the generated point mutation comprises that a base C is mutated into a base T or a base A. The found new mutation site can be used as a detection target applied in detection of the isoniazide resistance of mycobacterium tuberculosis, improves the molecular detection level of the isoniazide resistance of mycobacterium tuberculosis, shortens the diagnosis time of patients, and saves the treating time and cost for the patients. The mycobacterium tuberculosis drug-resistant mutation site is explained to possibly occur in a non-coding region, the base mutation of the intergenic region is prompted to be possibly related to the drug resistance, and a drug-resistant biomarker also exists. The invention provides a new idea for finding out a new and more effective mycobacterium tuberculosis drug-resistant molecular marker.
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Description

technical field

[0001] The invention relates to high-throughput screening and verification of drug-resistant base mutation sites of Mycobacterium tuberculosis, in particular to the screening and verification of isoniazid drug-resistant mutation sites of Mycobacterium tuberculosis. Background technique

[0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis infection. Mycobacterium tuberculosis may invade various organs of the human body, but mainly invades the lungs, which is called pulmonary tuberculosis. By the late 1980s, due to factors such as poverty, AIDS, population mobility, and drug resistance, the incidence and mortality of tuberculosis rose rapidly around the world, becoming the most serious infectious disease in the world alongside AIDS. The drug resistance of Mycobacterium tuberculosis is serious, and the drug resistance rate is as high as 46%, which poses a severe challenge to the prevention and treatment of tuberculosis. ...

Claims

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