Caenorhabditis elegans stress response behavior observation platform base and manufacturing method thereof

An observation platform, a technology for Caenorhabditis elegans, which is applied in animal husbandry and other directions, can solve the problems of inaccurate counting of nematodes toxicology test medium, large error in death judgment, inaccurate behavior observation, etc., and achieve shortened observation time, convenient observation, and efficiency. improved effect

Inactive Publication Date: 2014-06-25
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to solve the problems of inaccurate counting of the culture substrate of the existing nematode toxicology test, large error in death judgment, long observation time, and inaccurate behavior observation, and provides a substrate for the observation platform of the stress response behavior of Caenorhabditis elegans and its preparation method

Method used

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Effect test

specific Embodiment approach 1

[0016] Specific implementation mode 1: The base of a Caenorhabditis elegans stress response behavior observation platform in this embodiment is composed of 15% to 25% MgSO by mass percentage 4 , 15% to 25% of CaCl 2 , 3% to 15% agarose, 1% to 3% KCl, 1% to 2% alamar blue and 30% to 65% distilled water; the MgSO 4 and CaCl 2 The mass ratio is 1:1.

[0017] The observation platform of this embodiment has a blue-black culture substrate colored by Alma blue. Compared with the existing culture substrates such as agar and water liquid similar to the body color of nematodes, nematodes are obvious and observation is more convenient.

[0018] This embodiment enables live nematodes to stand vertically and violently wag their tails on a substrate with high hardness, while dead nematodes do not stand and swing, which makes the counting of nematodes and death determination intuitive and visible, and there will be no insect loss or leakage phenomenon, the observation time is shortened to...

specific Embodiment approach 2

[0020] Specific embodiment two: the difference between this embodiment and specific embodiment one is: by mass percentage, the MgSO of 18%~22% 4 , 18%~22% CaCl 2 , 5% to 10% agarose, 1.5% to 2.5% KCl, 1% to 2% Almar blue and 30% to 65% distilled water. Others are the same as the first embodiment.

specific Embodiment approach 3

[0021] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: by mass percentage by 16% MgSO 4 , 16% CaCl 2 , 6% agarose, 2% KCl, 1% alamar blue and 59% distilled water. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention relates to a caenorhabditis elegans stress response behavior observation platform base and a manufacturing method thereof. The problem that an existing elegans toxicity test cultivating medium is inaccurate in counting, large in death judging error, long in observation time and inaccurate in behavior observation is solved. The caenorhabditis elegans stress response behavior observation platform base comprises MgSO4, CaCl2, agarose, KCl, Alamar blue and distilled water according to mass percent. The method comprises the steps that weighed agarose and KCl are poured into distilled water for even stirring, and after heating boiling, cooling is carried out; MgSO4, CaCl2 and Alamar blue are added, and the pH value is adjusted through a KOH solution of 5 mol / L; the materials are poured into a 24-hole culture plate, and the liquid level height is 0.1 cm-0.2 cm; and the materials stand on a horizontal table until the materials are solidified, and the caenorhabditis elegans stress response behavior observation platform base is obtained. The caenorhabditis elegans stress response behavior observation platform base is mainly used for an existing elegans toxicity test.

Description

technical field [0001] The invention relates to a Caenorhabditis elegans stress response behavior observation platform substrate and a preparation method thereof. Background technique [0002] The elegans Caenorhabditiselegans belongs to the phylum Nematoda, which can sense changes in the surrounding environment more sensitively. Its life cycle is short, and it can indicate environmental changes in a short period of time. Because it is easy to cultivate and preserve, it is widely used as a model Biology, in experimental sciences such as toxicology, conducts behavioral observation research in the fields of environmental science and life science, and is used as a sensitive indicator organism for ecological and environmental health assessment. [0003] The C. elegans carrier for behavioral observation in toxicology tests is generally agar medium or test liquid. Caenorhabditis elegans has a transparent body with a body length of about 1 mm and can be seen under a microscope. C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 王云彪
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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