A method for preparing the substrate of a Caenorhabditis elegans stress response behavior observation platform

An observation platform, a technology for Caenorhabditis elegans, which is applied in animal husbandry and other directions, can solve the problems of inaccurate counting of nematode toxicology test medium, inaccurate behavior observation, large error in death judgment, etc., and achieves shortened observation time, convenient and accurate observation. The effect of increased accuracy and precision

Inactive Publication Date: 2015-12-30
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Application Information

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Problems solved by technology

[0005] The present invention aims to solve the problems of inaccurate counting of the culture substrate of the existing nematode toxicology test, large error in death judgment, long observation time, and inaccurate behavior observation, and provides a substrate for the observation platform of the stress response behavior of Caenorhabditis elegans and its preparation method

Method used

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specific Embodiment approach 1

[0016] Specific embodiment 1: The substrate of a Caenorhabditis elegans stress response behavior observation platform of this embodiment is composed of 15% to 25% MgSO by mass percentage 4 , 15%~25% CaCl 2 , 3% to 15% agarose, 1% to 3% KCl, 1% to 2% alamar blue and 30% to 65% distilled water; the MgSO 4 and CaCl 2 The mass ratio is 1:1.

[0017] The observation platform of the present embodiment has a blue-black culture substrate colored by alamar blue. Compared with the existing culture substrates such as agar and water, which are similar to the body color of nematodes, the nematodes are obvious and the observation is more convenient.

[0018] This embodiment enables live nematodes to stand vertically and violently wag their tails on a substrate with high hardness, while dead nematodes do not stand and swing, which makes the counting and death judgment of nematodes intuitively visible, and there will be no loss or leakage of worms. The observation time is shortened to less...

specific Embodiment approach 2

[0020] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the mass percentage of MgSO ranges from 18% to 22%. 4 , 18%~22% CaCl 2 , 5% to 10% of agarose, 1.5% to 2.5% of KCl, 1% to 2% of alamar blue and 30% to 65% of distilled water. Others are the same as the first embodiment.

specific Embodiment approach 3

[0021] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that: by mass percentage, 16% MgSO 4 , 16% CaCl 2 , 6% agarose, 2% KCl, 1% alamar blue and 59% distilled water. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a caenorhabditis elegans stress response behavior observation platform base and a manufacturing method thereof. The problem that an existing elegans toxicity test cultivating medium is inaccurate in counting, large in death judging error, long in observation time and inaccurate in behavior observation is solved. The caenorhabditis elegans stress response behavior observation platform base comprises MgSO4, CaCl2, agarose, KCl, Alamar blue and distilled water according to mass percent. The method comprises the steps that weighed agarose and KCl are poured into distilled water for even stirring, and after heating boiling, cooling is carried out; MgSO4, CaCl2 and Alamar blue are added, and the pH value is adjusted through a KOH solution of 5 mol / L; the materials are poured into a 24-hole culture plate, and the liquid level height is 0.1 cm-0.2 cm; and the materials stand on a horizontal table until the materials are solidified, and the caenorhabditis elegans stress response behavior observation platform base is obtained. The caenorhabditis elegans stress response behavior observation platform base is mainly used for an existing elegans toxicity test.

Description

technical field [0001] The invention relates to a preparation method of a Caenorhabditis elegans stress response behavior observation platform substrate. Background technique [0002] C. elegans Caenorhabditiselegans belongs to the Nematoda phylum. It can sensitively sense changes in the surrounding environment. It has a short life cycle and can indicate environmental changes in a short period of time. It is widely used as a model because it is easy to cultivate and preserve. Organisms are used to conduct behavioral observational research in the fields of environmental science and life science in experimental sciences such as toxicology, and are used as sensitivity indicator organisms for ecological and environmental health assessment. [0003] The C. elegans carrier used for the behavioral observation of the toxicology test is generally an agar medium or a test liquid. The body of C. elegans is transparent, and the body length is generally about 1mm, which can be seen unde...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
Inventor 王云彪
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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