Method for constructing transcription expression gene model of ultra-conserved region of uremic peripheral blood mononuclear cell, and application of model

A technology of ultra-conserved region and construction method, applied in the field of construction of transcription and expression of ultra-conserved region in peripheral blood mononuclear cells of uremia

Active Publication Date: 2014-07-23
中国人民解放军联勤保障部队第九二四医院
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Problems solved by technology

[0004] However, T-UCRs have not been studied in uremia

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  • Method for constructing transcription expression gene model of ultra-conserved region of uremic peripheral blood mononuclear cell, and application of model
  • Method for constructing transcription expression gene model of ultra-conserved region of uremic peripheral blood mononuclear cell, and application of model
  • Method for constructing transcription expression gene model of ultra-conserved region of uremic peripheral blood mononuclear cell, and application of model

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Embodiment Construction

[0018] In order to facilitate the understanding of the present invention, the present invention will be described in more detail below in conjunction with the accompanying drawings and specific embodiments. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described in this specification. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0019] Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used in the description of the present invention in this specification are only for the purpose of describing specific embodiments, and are not used to limit the present invention. The term ...

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Abstract

The invention relates to a method for constructing a transcription expression gene model of an ultra-conserved region of a uremic peripheral blood mononuclear cell, and an application of the model. The construction method comprises the following steps: arranging a uremia group and a normal control group, wherein each of the above groups comprises a plurality of whole blood specimens separate from different human bodies, and respectively separating to obtain the uremic peripheral blood mononuclear cell; determining the ribonucleic acid content, the mass and the integrity of the specimens; analyzing the transcripton expression level of the human body ultra-conserved region by adopting an ultra-conserved region transcripton chip, and detecting a gene specificity probe marked ultra-conserved region gene; carrying out ribonucleic acid marking and chip hybridization; analyzing the obtained chip scanning result; filtering a latent ultra-conserved region transcripton and a ribonucleic acid transcripton of the ultra-conserved region overlapping with the ultra-conserved region transcripton for dientifyign differential expression; comparing the multiple change among the specimen in each of the uremia group and the normal control group, and screening expression difference ribonucleic acid; and selecting ribonucleic acid with the expression difference exceeding a preset difference threshold to construct the gene model.

Description

【Technical field】 [0001] The invention relates to the field of uremia, in particular to a construction method and application of transcriptional expression of an ultra-conserved region of peripheral blood mononuclear cells in uremia. 【Background technique】 [0002] Uremia is not a single disease, but an autotoxic syndrome caused by the accumulation of metabolites and other toxic substances in the body due to renal failure, which is the most serious manifestation of renal failure. The morbidity and mortality of cardiovascular disease in patients with uremia are several times higher than that of ordinary people. In the current clinical practice, for the diagnosis of uremia, it is analyzed from clinical symptoms, laboratory tests or images. When chronic renal failure is at the end stage, renal replacement therapy, such as hemodialysis or kidney transplantation, is required, and renal In terms of replacement therapy, the determination of water-soluble small molecular substances...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N5/0786
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 眭维国戴勇曹翠辉薛雯
Owner 中国人民解放军联勤保障部队第九二四医院
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