Method for producing multi-antibody serum for resisting complement C3 by utilizing sheep raised in Zhejiang province

Abstract

The invention discloses a method for producing a multi-antibody serum for resisting a complement C3 by utilizing sheep raised in Zhejiang province. The method comprises the following steps: mixing 0.1ml of serum of an antigen (20mg) of the complement C3, 1.9ml of sterile PBS and 2ml of Freund's adjuvant and emulsifying so as to obtain an immunogen emulsion; then, injecting the immunogen emulsion into the sheep raised in Zhejiang province for the first time, i.e., injecting the immunogen emulsion into two areas of each front groin and two areas of each rear groin of each sheep for a total of 12 injection points in a subcutaneous injection manner and averagely injecting the immunogen emulsion in two points at the bottoms of feet of each sheep, then injecting the immunogen emulsion into the sheep for the secondary time at an interval of 20 days, and injecting the immunogen emulsion into the sheep for the third time to the sixth time at an interval of 10 days each time; and sampling blood from the artery of each immune sheep after an interval of 5 days, storing the blood in a water tank at 40 DEG C for 1 hour, and carrying out centrifugal sedimentation after the serum is fully separated out so as to separate out an antiserum. According to the method, the antigen of the complement C3 is subcutaneously injected into the outsides of the groins of the sheep raised in Zhejiang province, and the booster immunization is carried out for multiple times, so that the efficient sheep antibody capable of resisting the complement C3, which can reach a ratio above 1 to 64, is generated. Thus, a raw material for a biological reagent factory is provided, and the medical diagnosis can be conveniently carried out. As a result, the high benefit obtained from the sheep raised in Zhejiang province is achieved.

Description

technical field [0001] The invention relates to a method for producing goat anti-human serum, in particular to a method for producing anti-complement C3 polyantiserum by using Hu sheep and a method for using the same. Background technique [0002] C3 is the complement component with the highest content in serum, with a molecular weight of 195,000. It is mainly synthesized by macrophages and the liver. Under the action of C3 convertase, it is cleaved into two fragments, C3a and C3b. play an important role in both. C3 is an important raw material for biological reagents. The existing production method adopts the traditional immunization method, and the yield and potency are low, which can no longer meet the growing demand for use. Contents of the invention [0003] Purpose of the invention: In order to overcome the deficiencies of the above-mentioned prior art, the present invention provides a method for producing anti-complement C3 polyantiserum by using Hu sheep, which ca...

AI Technical Summary

Problems solved by technology

C3 is an important raw material for biological reagents. The existing production method adopts the traditional immunization method, and the yield and potency are low, which can no longer meet the growing demand for use.