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Quick determination method of artificial decenoic acid

A detection method, a qualitative technology of decenoic acid, used in measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2014-12-17
JIANGSU SINOGRAPHY TESTING
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  • Claims
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Problems solved by technology

[0004] The purpose of the present invention is to develop a method that can quickly and accurately treat the artificial decenoic acid in royal jelly and freeze-dried powder in view of the existing tricks of adding artificial decenoic acid into royal jelly and freeze-dried powder in the current market to obtain high profits. Qualitative and quantitative detection methods of alkenoic acid

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Embodiment Construction

[0015] 1. Pretreatment: Weigh 0.100g of the sample into a 25mL volumetric flask, dissolve in absolute ethanol, constant volume, ultrasonically extract for 30min, pass through a microporous membrane to be injected. At the same time, prepare the standard solution of artificial 10-HDA. (It is stipulated that 0.100g artificial 10-HDA standard substance is fixed in a 25mL volumetric flask, and the concentration of artificial decenoic acid is 100%)

[0016] 2. Preparation of the standard solution of artificial 10-HDA: Accurately weigh 0.100g of the standard artificial 10-HDA, set the volume in a 25mL volumetric flask with absolute ethanol, and then dilute the standard solution with absolute ethanol to 0.05 %, 0.1%, 0.2%, 0.5%, 1.0%, 2.0% and 5.0% six standard points to establish a standard curve.

[0017] 3. Quantification by external standard method, the standard curve equation is Y=-3.223E3+7.759E5*X, R2=0.999.

[0018] 4. Repeatability experiment of detection method: add artifi...

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Abstract

The invention belongs to the technical field of food detection, in particular relates to a quick and accurate method for detecting whether pure natural royal jelly and freeze-dried powder are mixed with artificial decenoic acid, and aims to develop a quick and accurate method for qualitative and quantitative detection of the artificial decenoic acid in the royal jelly and the freeze-dried powder in allusion to the foul means of mixing of the artificial decenoic acid in the royal jelly and the freeze-dried powder for seeking high profits in the market in the prior art. The invention discloses the quick determination method of the artificial decenoic acid in the royal jelly and the freeze-dried powder so as to achieve the purpose, and the quick determination method comprises three steps of pretreatment, liquid chromatographic separation and mass spectrometric detection. According to the HPLC-MS detection method, ion peaks with the mass to charge ratio of 225 m / z (parent ion), 163 m / z (daughter ion) and 181 m / z (daughter ion) are taken as feature detection peaks.

Description

technical field [0001] The invention belongs to the technical field of food detection, and in particular relates to a rapid detection method for whether artificial decenoic acid is mixed in pure natural royal jelly and freeze-dried powder. Background technique [0002] Decenoic acid, namely 10-hydroxy-α-decenoic acid, also known as royal jelly acid, or 10-HDA for short, is a unique and highly effective biologically active substance contained in royal jelly, so it is a marker of royal jelly. Pure natural royal jelly generally contains 1.4% to 2.0% decenoic acid. The national standard stipulates that the 10-HDA content in royal jelly reaches 1.4% as a qualified product, and when it reaches 1.8%, it is a superior product; the 10-HDA content in the freeze-dried powder reaches 5.0% as a first-class product, and when it reaches 4.0%, it is a second-class product. The level of 10-HDA content directly affects the price of the product. Therefore, some unscrupulous traders add artif...

Claims

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Application Information

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IPC IPC(8): G01N30/06
Inventor 徐锦忠王燕杨波王英俊刘秀红王祝超
Owner JIANGSU SINOGRAPHY TESTING
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