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A kind of tissue culture rapid propagation method of Eucalyptus urophylla dh32-29 variety

A tissue culture rapid propagation and variety technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of inability to effectively inherit the excellent characteristics of the parents, the tissue culture work has not been carried out effectively, and the excellent characteristics need to be studied. Achieve the effect of improving economic and social benefits, easy to popularize and use, and reducing the risk of tissue culture

Active Publication Date: 2016-04-06
广西八桂种苗高科技集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above-mentioned patent uses the stem section of Eucalyptus urophylla aseptic seedlings as explants to induce embryogenic callus, and then obtains regenerated plants through the regeneration of somatic embryogenesis. The operation is relatively complicated and difficult to implement. The stems of the seedlings are explants, and the excellent characteristics of the female parent need to be studied. The commercial seedling cultivation using this technology alone cannot effectively inherit the excellent characteristics of the parents.
[0009] It can be seen that although there are many studies on the tissue culture technology of eucalyptus at present, there is still a lot of work to be explored for the purposeful propagation of the excellent traits of the excellent trees, and the tissue culture rapid propagation method that can be effectively popularized and applied. Due to the DH32-29 It is a perennial species of Eucalyptus urophylla. There are few tissue-cultured seedlings in the market and resources are in short supply. However, the tissue culture of this variety has not been effectively carried out. The tissue culture research on the new variety of Eucalyptus urophylla DH32-29 is helpful for the rapid development of this fine variety. It is of great significance to breed, meet the market supply, and improve the ecological and economic benefits of this fine variety

Method used

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  • A kind of tissue culture rapid propagation method of Eucalyptus urophylla dh32-29 variety

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Tail Eucalyptus DH32-29 varieties of varieties are fast-growing methods. The specific steps are as follows:

[0033] ① The establishment of outer implants: Choose 4-5 years old age, strong growth, large trunk, and disease-free and insect pests. It falls from 20-25cm from the ground.When germinated, the buds are returned as an outer plant material.In order to reduce the source of material pollution, when bud strips are adopted, it should be carried out for more than three consecutive days of clear weather.After removing most of the buds, rinse it with tap water for 15 minutes, soak the 0.5%laundry powder solution for 15 minutes, and then rinse it with tap water.

[0034] ② External implant disinfection: Step ① The obtained outer implant is soaked in 75 % alcohol for 30s, washed 5 times of sterile water, drained the water, and then placed in a 0.1 % mercury solution for 9 minutes.Second, cut the buds in the second quarter of the budding bud, 1-2 cm, 1-2cm long.

[0035] ③ The...

Embodiment 2

[0041] Tail Eucalyptus DH32-29 varieties of varieties are fast-growing methods. The specific steps are as follows:

[0042] ① The establishment of outer implants: Choose 4-5 years old age, strong growth, large trunk, and disease-free and insect pests. It falls from 20-25cm from the ground.When germinated, the bud bar is used as an external implant material.In order to reduce the source of material pollution, when bud strips are adopted, it should be carried out for more than three consecutive days of clear weather.After removing most of the buds, rinse it with tap water for 15 minutes, soak the 0.5%laundry powder solution for 15 minutes, and then rinse it with tap water.

[0043] ② Expuel disinfection: Step ① The obtained outer implant is soaked in 75 % alcohol for 30s, washed 4 times of sterile water, drained the water, and then placed in a 0.1 % mercury solution for 9 minutes.Second, cut the buds in the second quarter of the budding bud, 1-2 cm, 1-2cm long.

[0044] ③ The first ...

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Abstract

The invention discloses a tissue culture and rapid propagation method of Eucalyptus urophylla DH32-29 variety, which is realized through the steps of explant establishment, explant disinfection, primary culture, subculture, rooting culture, seedling hardening and transplanting. , A special medium composition was selected for primary culture, subculture and rooting culture. Reasonable seedling hardening measures made Eucalyptus macrophylla grow fast after transplanting, with a high survival rate, and achieved the purpose of high reproduction coefficient and good reproduction effect. Simultaneously, the production cost of the method of the invention is low, and the obtained tissue culture seedlings have consistent genetic traits, which is an effective way for tissue culture and rapid propagation of eucalyptus, and has a wide application range.

Description

Technical field [0001] The present invention involves the field of eucalyptus cultivation technology, and specially involves a fast-growing method of a variety of Eucalyptus DH32-29 varieties. Background technique [0002] Eucalyptus is the collective name of the Eucalyptus plant. It has produced more than 600 varieties after many years in Australia.About 100 kinds, more than 40 types of extensive cultivation.In recent years, all parts of my country are developing eucalyptus cultivation, forming a certain scale in provinces such as Guangdong, Guangdong, Hainan, Sichuan, Yunnan, etc., and have produced obvious ecological and economic benefits. [0003] Eucalyptus DH32-29 variety ( E.urophylla × E.Grandis) , It is the east gate of Guangxi, with Indonesia's Source East Gate Eucalyptus Eucalyptus U16 ( EUCALYPTUSUROPHYLLA ) As a mother -based, with the East Gate of the East Gate of Australia, G46 ( EUCALYPTUSGRANDIS ) As a variety of eucalyptus varieties obtained by non -sexual breed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCY02A40/22Y02P60/40
Inventor 庞贞武沈云黄全东吴兵梁秀莉陈允椿韦炳俭吴满芬俸荣娣邓冬丽付淼吕华丽莫继有兰俊唐再生吴永富龚卫新
Owner 广西八桂种苗高科技集团股份有限公司
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