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A promoter capable of driving or regulating gene expression in plant stamens

A technology for regulating genes and promoters, which is applied in the field of gene fragments, can solve the problems of no crops and stamen-specific expression of limited promoter types, etc., and achieve the effects of improving growth characteristics and mechanisms, improving growth characteristics and mechanisms, and improving flower types

Active Publication Date: 2017-02-22
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the types of stamen-specific expression promoters currently known to people are limited, and, as mentioned above, the existing stamen promoters are mainly suitable for improving the ornamental quality of flowers, and there are not enough promoters that can be applied to Stamen promoters in crops, especially monocotyledonous crops, especially rice

Method used

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  • A promoter capable of driving or regulating gene expression in plant stamens
  • A promoter capable of driving or regulating gene expression in plant stamens
  • A promoter capable of driving or regulating gene expression in plant stamens

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Embodiment Construction

[0040] The present invention will be described below with reference to specific examples.

[0041] 1. Plant material

[0042] The mature seeds of Nipponbare rice were preserved by the Biotechnology Office of the Rice Institute of Anhui Academy of Agricultural Sciences.

[0043] 2. Strains and plasmids

[0044] The Escherichia coli strain used in the present invention is XL1-blue; Agrobacterium tumefaciens is EHA105, which is preserved in the Biotechnology Office of the Rice Institute of Anhui Academy of Agricultural Sciences; the plant expression vector pCAMBIA1391 is purchased from CAMBIA Company of Australia. Of course, those skilled in the art should understand that similar effects can also be achieved by using other bacterial strains or expression vectors, which are not limited here.

[0045] 3. Reagents and medicines

[0046] Sodium hypochlorite (NaClO, available chlorine concentration 4%), Tween20 was purchased from Sigma Company. Hygromycin B was purchased from Roch...

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Abstract

The present invention provides a promoter capable of driving or regulating gene expression in plant stamens, which comprises at least one of the following: (a) the nucleotide sequence shown in SEQ ID NO1; (b) the nucleotide sequence shown in SEQ ID NO2 The nucleotide sequence shown; (c) the nucleotide sequence obtained after adding, replacing or missing one or more nucleotides in the nucleotide sequence shown in SEQ ID NO1; (d) in SEQ ID NO1 The nucleotide sequence obtained after adding, substituting or missing one or more nucleotides in the nucleotide sequence shown in ID NO2; (e) a complementary core to the nucleotide sequence shown in SEQ ID NO1 Nucleotide sequence; (f) a nucleotide sequence complementary to the nucleotide sequence shown in SEQ ID NO2; (g) a nucleotide sequence with at least 90% homology to SEQ ID NO1; or (h) A nucleotide sequence having at least 90% homology to SEQ ID NO2. The promoter of the present invention is beneficial to control the specific expression of the target gene only in stamens.

Description

technical field [0001] The invention relates to a gene fragment with specific functions isolated from plants, in particular to a stamen-specific expression promoter isolated from rice. Background technique [0002] One of the goals of plant genetic engineering is to produce plants with characteristics or traits desired for agriculture. With the advancement of genetic engineering, the necessary tools for transforming plants to contain and express foreign genes are now available. Technological advances in plant transformation and regeneration have enabled researchers to obtain exogenous nucleotide molecules and integrate these nucleotide molecules into plant genomes. The exogenous nucleotide molecule can then be expressed in the plant cell to exhibit the added characteristic or trait. Therefore, plants, especially crops, can be provided with some traits that the crops do not have originally, so that the growth mode of the crops is more in line with the needs of people's prod...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/82C12N1/21A01H5/00
Inventor 魏鹏程杨剑波秦瑞英杨亚春李浩李莉马卉许蓉芳
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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