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Method for promoting the massive accumulation of high unsaturated fatty acids in Nannochloropsis

An unsaturated fatty acid and Nannochloropsis technology is applied in the field of promoting the massive accumulation of highly unsaturated fatty acids by Nannochloropsis, can solve the problems of low temperature resistance, poor stress resistance, and difficulty in large-scale popularization and application, and achieves low pollution, Low cost and the effect of avoiding light damage

Active Publication Date: 2018-10-16
临沂东旭食品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the algae strain has poor stress resistance and low temperature tolerance, so it is difficult to apply it on a large scale.

Method used

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  • Method for promoting the massive accumulation of high unsaturated fatty acids in Nannochloropsis
  • Method for promoting the massive accumulation of high unsaturated fatty acids in Nannochloropsis
  • Method for promoting the massive accumulation of high unsaturated fatty acids in Nannochloropsis

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Embodiment Construction

[0015] The preparation method of the soil leach solution described in step 1: take 1 kg of loose, organic matter-rich upper soil in the woods, add 5 liters of tap water, boil for 20 minutes, pour the mud into a beaker, let it stand for 24 hours, and then absorb the supernatant , pour it into a Erlenmeyer flask, add a cotton plug, boil for 15 minutes, cool, let it settle for 12 hours, take the supernatant to obtain the soil leaching solution, put it into a reagent bottle with a ground stopper, seal it, label it, and place it at 4°C Refrigerator spare;

[0016] Ferric citrate (FeC) described in step 1 6 h 5 o 7 ) is difficult to dissolve, you can add a small amount of tap water and heat it slightly on the stove to 80-90°C, and keep stirring until it completely melts;

[0017] The preparation method of sheep manure leachate described in step 1: pile up sheep manure in the sun, expose to the sun properly, so that the water content of sheep manure is preferably 50-75%, seal and ...

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Abstract

The invention discloses a method for promoting nannochloropsis oculata to massively accumulate high unsaturated fatty acids, belonging to the field of aquaculture. Conventionally, the nannochloropsis oculata which is cultured by using a large open track pool is slow to grow, troublesome to operate, easy to pollute and low in content of high unsaturated fatty acids. The method adopts a second-stage culture method. The first stage is carried out in an illumination incubator, and relatively low temperature and illumination intensity are provided, thereby facilitating growth of algae cells and preventing the cells from being damaged by hardlight. The second stage is carried out in a microalgae culture chamber, in which a plastic thin film bag is used for inflated culture, so that the temperature and illumination are improved, thereby facilitating accumulation of the high unsaturated fatty acids. According to the method disclosed by the invention, the conventional nutrient solution formula is also optimized and supplemented by human urea, KBr, H3OB3, nicotinic acid, pyridoxine hydrochloride, soil lixivium and the like, so that the nutrient solution formula is comprehensive and balanced in nutrients, hard to pollute, low in cost and easy to operate, and the content of the high unsaturated fatty acids is improved by 140%.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a method for promoting Nannochloropsis to accumulate a large amount of highly unsaturated fatty acids. Background technique [0002] Nannochloropsis oculata, also known as Nannochloropsis oculata, is a eukaryotic single-celled green microalgae with a diameter of 2-3 μm, belonging to the Chlorophyta, Chlorophyceae, Tetracellulares, Gum Chlorellaceae, Nannochloropsis genus. The oil content in the cell accounts for more than 68% of the dry weight, and the oil is mainly composed of C16 and C18 fatty acids. It is recognized as the most promising marine microalgae for industrial refining of biodiesel. In addition, the algae is also rich in high unsaturated fatty acid EPA, eicosapentaenoic acid. EPA has important physiological and health functions for the human body, and can assist in the prevention and treatment of heart disease, arteriosclerosis, cancer, asthma, rheumatoid arth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6427
Inventor 王培磊
Owner 临沂东旭食品有限公司
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