Preparation method of glutathione-modified nano-gold colorimetric probe and method for detecting trivalent chromium ions

A glutathione and colorimetric probe technology, applied in the field of chromium ion detection, can solve the problems of unsuitable on-site batch detection, reagent pollution environment, weak stability, etc., and achieve no toxic side effects, good selectivity, and stability Good results

Inactive Publication Date: 2017-12-22
HUAZHONG AGRI UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

These commonly used analytical methods have the advantages of high accuracy and high sensitivity, but often require expensive instruments and equipment, complicated operation methods, long-term pretreatment process, and the reagents used in the detection process pollute the environment, and are not suitable for large-scale On-site batch testing and analysis
Therefore, some rapid detection methods have emerged at present, such as enzyme inhibition method and enzyme-linked immunoassay, but enzymes are used in these methods, resulting in the disadvantages of weak stability, poor reproducibility, and high cost of these detection methods
[0004] According to relevant literature, the method of detecting trivalent chromium ion using glutathione-modified nano-gold probe has not been reported yet.

Method used

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  • Preparation method of glutathione-modified nano-gold colorimetric probe and method for detecting trivalent chromium ions
  • Preparation method of glutathione-modified nano-gold colorimetric probe and method for detecting trivalent chromium ions
  • Preparation method of glutathione-modified nano-gold colorimetric probe and method for detecting trivalent chromium ions

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preparation example Construction

[0046] A method for preparing a glutathione-modified nano-gold colorimetric probe, comprising the following steps:

[0047] 1) Heat the chloroauric acid solution at 95-110°C, then add the citric acid solution, stir for 10-30 minutes, and then stir at room temperature for 10-20 minutes; after standing at room temperature, the nano-gold solution is obtained; keep it away from light at 4°C for later use ; Wherein, the molar ratio of chloroauric acid and citric acid is 10: 38.8; Wherein, the color of nano-gold solution is wine red;

[0048] 2) Add glutathione solution to the nano-gold solution prepared in step 1), place it in a constant temperature shaker at 25°C and shake it for 1-2 hours. Centrifuge for 10-30 minutes under the same conditions, remove the supernatant, add ultrapure water to dissolve the precipitate at the bottom of the tube; repeat the centrifugation once, remove the supernatant, and re-dissolve the precipitate to obtain the glutathione-modified gold nanometer co...

Embodiment 1

[0057] Add 100mL of 1mM chloroauric acid solution to a 250mL round-bottom two-neck flask (soak overnight in fresh aqua regia, wash and dry with ultrapure water) and heat in a water bath at 98°C, then quickly add 10mL of 38.8mM citric acid solution, and stir vigorously for 10min. The black color turned into wine red, and then stirred at room temperature for 15 minutes, and then kept at room temperature and protected from light at 4°C for later use.

[0058] Mix 4 mL of the above-prepared nano-gold solution with 2 mL of ultrapure water, and then add 600 μL of 1×10 -4 M Freshly prepared glutathione solution, shake in a constant temperature shaker at 25°C for 1h, take it out and stand still for 4h, then centrifuge at 14000rmp at 4°C for 25min, remove the supernatant, and dissolve the precipitate at the bottom of the tube with ultrapure water , repeat the centrifugation once, remove the supernatant, redissolve the precipitate and store it in the dark at 4°C for later use. Measure ...

Embodiment 2

[0060] Prepare standard curve according to the glutathione-modified nano-gold colorimetric probe prepared in Example 1, the specific method is as follows:

[0061] Prepare ultrapure aqueous solutions of different concentrations of trivalent chromium ions (concentrations of trivalent chromium ions are 0.02mg / L, 0.08mg / L, 0.3mg / L, 0.5mg / L, 1mg / L respectively), take 100μl glutathione Add 0.02M Nacl solution with a volume of 20 μl to the glycide-modified nano-gold solution, mix well and then add 120 μl ultrapure aqueous solutions of trivalent chromium ions with different concentrations, measure the visible absorption spectrum of the mixed solution after 10 minutes, and use A 700 / A 520 The value of the chromium ion concentration is the ordinate, and the value of the chromium ion concentration is the abscissa to draw the standard curve (attached Figure 4 ), adding the glutathione-modified A of the nano-gold colorimetric probe solution of different concentrations of trivalent chrom...

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Abstract

The invention discloses a preparation method of a glutathione-modified nano-gold colorimetric probe and a method for detecting trivalent chromium ions. The preparation method firstly heats the chloroauric acid solution at 95-110° C., and then adds the citric acid solution to obtain the nano-gold solution; adds the glutathione solution to the prepared nano-gold solution, and shakes the reaction in a constant temperature shaker, Centrifuge after standing and remove the supernatant to obtain the glutathione-modified nano-gold colorimetric probe, get the glutathione-modified nano-gold colorimetric probe prepared above, add sodium chloride solution, mix evenly, Obtain a mixed solution; add the same volume of the sample solution to be tested into the mixed solution, let it stand for 10-20 minutes, observe the color change of the reaction solution or detect the visible absorption change of the mixed solution, and qualitatively and quantitatively analyze the existence or content of trivalent chromium ions. The nanometer probe prepared by the invention has good selectivity, high sensitivity and good stability, and can reach the detection level of the national standard.

Description

technical field [0001] The invention relates to the technical field of chromium ion detection, in particular to a method for preparing a glutathione-modified nano-gold colorimetric probe and a method for detecting trivalent chromium ions. Background technique [0002] Heavy metals generally refer to metals with a specific gravity greater than 4 or 5, and there are about 45 kinds, such as copper, lead, zinc, iron, cobalt, nickel, manganese, cadmium, mercury, tungsten, molybdenum, gold, silver, etc., heavy metals in environmental pollution Mainly refers to the biologically toxic metal elements such as mercury, cadmium, lead, chromium and metalloid arsenic. The intensification of heavy metal pollution in the environment leads to excessive heavy metal content in agricultural products, which poses an increasing danger to human health. Heavy metal pollutants are difficult to degrade in the environment, difficult to metabolize in the organism and can accumulate in the organism, gra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 刘骞徐胜臻陈长水李雪刚李建洪
Owner HUAZHONG AGRI UNIV
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