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A Strain of Chlamydocetes sp. sp. and its Screening and Application

A technology of Puconia spores and spores, applied in the fields of Chlamydobacterium chlamydoides Pekonia strains and its screening and application, can solve the problem of lack of strain resources for high-efficiency production of nematocides, and achieve the purpose of suppressing the number of offspring populations , the effect of inhibiting the reproduction of pests

Active Publication Date: 2017-08-22
YUNNAN UNIV
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Problems solved by technology

[0007] An object of the present invention is to overcome the deficiencies in the existing nematicides and the shortage of strain resources for high-efficiency production of nematicides, and to screen out the nematicide-producing Auranthus aureus from fungi with the function of poisoning and killing plant pathogenic nematodes Pochoniachlamydosporia (Pochoniachlamydosporia) YMF 1.00613, a strain of aurovertins secondary metabolites, lays the foundation for the research and development of biological nematicides and biomimetic biopesticides

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  • A Strain of Chlamydocetes sp. sp. and its Screening and Application
  • A Strain of Chlamydocetes sp. sp. and its Screening and Application
  • A Strain of Chlamydocetes sp. sp. and its Screening and Application

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Embodiment Construction

[0024] The Chlamydobacterium chlamydosporia (Pochonia chlamydosporia) YMF 1.00613 used in this example was deposited on May 10, 2010 in the General Microorganism Center of China Committee for the Collection of Microorganisms, and the preservation number is CGMCC-3806.

[0025] The screening steps of the nematicide fungus Chlamydosporia chlamydosporia (Pochonia chlamydosporia) YMF1.00613 strain used in this embodiment are as follows:

[0026] (1) Separation and purification of strains

[0027] Root-knot nematode-infected tobacco root samples were collected from the waterpipe field in Jianshi, Yunnan, and sealed with plastic bags. Cut ±300g root knots into 3cm or 5cm sections, put them in a Baermann funnel device filled with water, connect a rubber tube below the funnel to the nematode receiving test tube, and the nematode will sink to the bottom of the tube due to gravity. After 24 hours, clamp the rubber tube, remove the receiving tube, and centrifuge at 8000 rpm to discard t...

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Abstract

The invention relates to a strain of chlamydocetes sporoconiae and its screening and application, and belongs to the technical field of nematicidal biological resources and microbial strains. The Chlamydobacterium chlamydosporia (Pochonia chlamydosporia) YMF 1.00613 was deposited on May 10, 2010 in the General Microorganism Center of China Committee for the Collection of Microorganisms, with the preservation number CGMCC No.3806. The screening step of the bacterial strain includes: separation and purification of the bacterial strain, and screening of secondary metabolites of aurovertins produced by the bacterial strain. The application of the chlamydobacterium Pochonia chlamydosporia YMF 1.00613 strain of the invention in the preparation of agricultural insecticides for preventing and controlling plant pathogenic nematodes and the fermentation production of biological pesticides. The invention has the effect of significantly suppressing the reproduction of pests and the number of offspring populations. The strain PDB fermentation broth and its secondary metabolites, mainly composed of auromycin compounds, have the ability to kill nematodes, and the nematicide activity can reach more than 90% under experimental conditions, and can be used as biologically active substances such as nematicides and biological pesticides fermentation production.

Description

[0001] Technical field: [0002] The invention relates to a strain of chlamydocetes sporoconiae and its screening and application, and belongs to the technical field of nematicidal biological resources and microbial strains. [0003] Background technique: [0004] Phytopathogenic nematodes are a serious plant disease. According to reports, there are more than 5,000 species of phytopathogenic nematodes in more than 200 genera. Plant pathogenic nematode diseases cause economic losses of nearly 157 billion US dollars per year to agriculture worldwide, second only to fungal diseases. At present, chemical control is still one of the important measures to control plant nematodes, but the long-term use of highly toxic chemical nematicides is increasingly harmful to the environment and human health. Therefore, the research and development of bio-nematicides with high efficiency and low toxicity has become an important issue for the sustainable development of agriculture. [0005] The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P5/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 张克勤王彦利牛雪梅
Owner YUNNAN UNIV
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