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A Toxicity Evaluation Method of Haze Pollutants

An evaluation method and pollutant technology, applied in the field of environmental toxicology, can solve the problems of difficult operation, low accuracy, and single evaluation content, and achieve the effects of great flexibility, convenient material collection, and exclusion of influence

Inactive Publication Date: 2017-09-26
HANGZHOU NORMAL UNIVERSITY
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  • Claims
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Problems solved by technology

[0006] The purpose of the present invention is to solve the deficiencies of the existing atmospheric particulate matter cytotoxicity evaluation method, and propose a haze pollutant toxicity evaluation method, which can make up for the existing evaluation method. Single, low accuracy and other disadvantages

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  • A Toxicity Evaluation Method of Haze Pollutants
  • A Toxicity Evaluation Method of Haze Pollutants
  • A Toxicity Evaluation Method of Haze Pollutants

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Embodiment Construction

[0027] The present invention is further analyzed below in conjunction with specific examples.

[0028] As shown in the figure, the inventive method comprises the following steps:

[0029] Step (1). Inoculate the RTE rat tracheal epithelial cell line into the F12 culture medium containing fetal bovine serum, and place it in a sterile cell incubator at 37°C for 3 to 4 days; inoculate the cultured cells into the culture medium dish, inoculate 5×10 per dish 6 ~8×10 6 rat tracheal epithelial cells;

[0030] The F12 culture solution containing fetal bovine serum contains fetal bovine serum with a volume content of 3-7%, preferably a volume content of 5%;

[0031] The petri dish has a diameter of 5 cm and a height of 1 cm, and needs to be sterilized before use;

[0032] Step (2). Then in step (1), the cell culture dish is placed in the atmospheric particle collection device placed in the cell culture medium in the haze area, and the cells are poisoned at the temperature required ...

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Abstract

The invention discloses a method for evaluating the toxicity of haze pollutants. The method is to inoculate and culture the RTE rat tracheal epithelial cell line, and inoculate it into a culture dish; place the cell culture dish in a cell culture medium atmospheric particle collection device to infect the cells; at the same time, collect the particles in the haze area, Detect the particle size, heavy metal concentration, and other organic and inorganic components, and detect the concentration of PM10 and PM2.5 in the air; put the poisoned cells into the cell impedance measuring instrument, and test their cell recovery ability, chemotaxis migration ability and Anti-invasion ability; comprehensive evaluation and comparison of the toxic effects of different types of haze particles on rat tracheal epithelial cells in different haze areas. Compared with the traditional toxicity evaluation method, the ECIS technology used in the present invention has greater flexibility, the obtained data changes in real time, and the meaning is richer; the material is convenient, and the influence of individual differences on the experiment can be eliminated, and no ethics are involved. question.

Description

technical field [0001] The invention belongs to the field of environmental toxicology and relates to a method for evaluating the toxicity of haze pollutants. Background technique [0002] In recent years, smog incidents have occurred frequently in China. Studies have shown that there is a positive correlation between smog and respiratory diseases, and every 10ug / m increase in PM2.5 in Shanghai 3 , the day and lag 1d (lag0 ~ 1) respiratory disease mortality increased by 0.95%. The main components of smog are sulfur dioxide, nitrogen oxides and inhalable particulate matter. Among them, inhalable particles are particles with a particle size between 2.5 and 10 microns. These particles can enter the upper respiratory tract, especially particles with a particle size less than or equal to 2.5 microns. Most of them can pass through the human bronchus, reach the lungs, and even enter the human blood circulation , leading to the occurrence of respiratory diseases. Therefore, under...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/02
Inventor 张杭君方文迪王彬浩曾少媛
Owner HANGZHOU NORMAL UNIVERSITY
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