Inducing agent and medium for inducing directional differentiation of embryonic stem cell into keratinocytes

Abstract

The invention provides an inducing agent for inducing directional differentiation of an embryonic stem cell into keratinocytes. The inducing agent comprises bone morphogenetic protein 4, retinoic acid and ascorbic acid. Preferably, in the inducing agent, the bone morphogenetic protein 4, retinoic acid and ascorbic acid are in a content ratio of (1-50g):(0.1-5 mol):(50-600mol). The inducing agent for inducing directional differentiation of the embryonic stem cell into keratinocytes combines the bone morphogenetic protein 4, retinoic acid and ascorbic acid, can effectively improve the efficiency for induced directional differentiation of hESC into keratinocytes (as high as 77.93%, and far higher than other formula inducing agents), provides an approach for efficiently inducing differentiation of hESC into keratinocytes, and provides seed cells for constructing tissue engineered oral mucosas.

Description

technical field [0001] The invention relates to an inducer for inducing the directional differentiation of embryonic stem cells, in particular to an inducing agent, culture medium and method for inducing the directional differentiation of embryonic stem cells into keratinocytes. Background technique [0002] The oral mucosa is the first barrier for the human body to resist various harmful factors from the outside world. It has very important functions such as protecting various tissues, sensations, temperature regulation and secretion under the oral mucosa. Oral mucosal tissue reconstruction is often required because periodontal surgery, pre-denture surgery, dental implant surgery, congenital or acquired facial deformity repair surgery, trauma, and tumor resection may cause oral mucosal tissue defect or insufficiency. Currently, autologous skin grafts or oral mucosal flaps are widely used to repair defects in clinic. However, after many years of skin transplantation, the or...

AI Technical Summary

Problems solved by technology

However, after many years of skin transplantation, the original structure and function of the skin epithelium and the characteristics of keratinization, secretion, and hair growth are still maintained, and it lacks the moist and smooth feeling of normal mucous membranes, which makes patients feel uncomfortable and is far from meeting the clinical needs, thus limiting clinical application.

And above-mentioned method all will open up the second operation field, will bring new trauma and misery to patient.

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