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Fingerprint spectrum common mode construction and quality detection method for epimedium herb wall-breaking decoction pieces

A technology of broken-walled decoction pieces and fingerprints, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to fully reflect the quality, specificity, stability, reproducibility and precision of decoction pieces, and achieve simple operation, Detect fast and accurate effects

Active Publication Date: 2015-08-05
ZHONGSHAN ZHONGZHI PHARMA GRP
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are detection methods involving fingerprints of traditional Chinese medicine in the prior art, there is no method for breaking the wall of Chinese medicine with special morphology, and there are defects in specificity, stability, reproducibility and precision, which cannot fully reflect the quality of the decoction pieces Defects

Method used

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  • Fingerprint spectrum common mode construction and quality detection method for epimedium herb wall-breaking decoction pieces
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  • Fingerprint spectrum common mode construction and quality detection method for epimedium herb wall-breaking decoction pieces

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Embodiment Construction

[0019] 1. The construction of HPLC standard fingerprint of Epimedium broken wall decoction pieces.

[0020] The present invention uses icariin, epimedin A, epimedin B, epimedin C, curariin B, icariside I, epimedin II and chlorogenic acid as 8 main active ingredients As the goal, establish a standard fingerprint that can more comprehensively reflect the composition information of Epimedium broken wall decoction pieces, and can also determine the content of icariin.

[0021] 1 Instruments and reagents

[0022] 1.1 Instrument

[0023] Agilent 1200RRLC fast liquid chromatograph (equipped with G1315C model DAD detector, G4218A ELSD evaporative light detector, G1312B binary pump, G1329B autosampler, G1322A degasser, G1316B column thermostat, Agilent Chemstation chromatography workstation) , 1 / 10,000 electronic analytical balance, 1 / 100,000 electronic analytical balance, high-power numerical control ultrasonic cleaner, etc.

[0024] 1.2 Reagent

[0025] Reagents: methanol (Oceanp...

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Abstract

The invention relates to a fingerprint spectrum common mode construction and quality detection method for epimedium herb wall-breaking decoction pieces. The method includes: taking icariin as control, under the chromatographic conditions of a column temperature of 35DEG C, a wavelength of 272nm, a phase A of acetonitrile, a phase B of water, an elution gradient of 0min-20min-28min-60min, and phase A change of 20%-27%-27%-50%, conducting HPLC analysis on over 10 batches of samples, establishing a common mode standard spectrum and judgment indexes, comparing the spectrum of a to-be-detected sample under the same chromatographic conditions with the standard spectrum to detect the quality of the to-be-detected sample. The invention relates to an HPLC common mode standard spectrum established directed to epimedium herb wall-breaking decoction pieces and a quality detection method. The spectrum comprehensively covers the spectrum information of epimedium herb wall-breaking decoction pieces' main active components, has strong specificity, is rapid and accurate in detection, and can effectively evaluate the overall quality of drugs, wall-breaking powder and wall-breaking decoction pieces.

Description

technical field [0001] The invention relates to a method for establishing and detecting traditional Chinese medicine decoction pieces, in particular to building a common pattern of HPLC fingerprints of broken-walled decoction pieces of Epimedium herba and its quality detection method. Background technique [0002] Epimedium is the dried leaves of Epimedium brevicornu Maxim, Epimedium sagittatum (Sie.et Zucc.), Epimedium pubescens Maxim. or Epimedium koreanum Nakai of Berberidaceae plants. Epimedium in the present invention is aimed at the species of Epimedium brevicornu Maxim of the Berberidaceae plant. [0003] The main components are flavonoids, lignans, alkaloids and polysaccharides. The flavonoids mainly include: icariin, epimedin A, epimedin B, epimedin C, baohuoside Ⅰ, Ⅱ, icariside Ⅰ, Ⅱ, icariin A, Rhamnosyl icariin Ⅱ, arrowiside A, B, C and hyperin, etc. [0004] Traditional Chinese medicine wall-broken decoction pieces use modern ultrafine pulverization technology...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 徐吉银王慧玲彭丽华成金乐
Owner ZHONGSHAN ZHONGZHI PHARMA GRP
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