Method of in-vitro measuring obesity-causing risk

An obesity and risk technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of no significant correlation, inaccuracy, different correlations, etc., to increase the risk of obesity Effect

Active Publication Date: 2015-08-12
TCI GENE
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Problems solved by technology

However, the results of the European study found that the variation of SDC3 was not significantly related to the occurrence of obesity. Furthermore, the association between the SNP sites of each SDC3 and the occurrence of obesity was different.
[0006] Based on the above, it may still be controversial to use PPARG or SDC3 polymorphism alone as an obesity genetic indicator. Specifically, it may still be inaccurate to use PPARG or SDC3 polymorphism alone to detect the risk of obesity

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  • Method of in-vitro measuring obesity-causing risk
  • Method of in-vitro measuring obesity-causing risk
  • Method of in-vitro measuring obesity-causing risk

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Embodiment

[0045] Materials and Methods

[0046]

[0047] This trial invites adults from Taiwan to participate through the Family Medicine Department and Community Medicine Center of Mackay Memorial Hospital, and excludes those who are currently pregnant, cancer, secondary obesity, obesity caused by congenital chromosomal abnormalities (such as Prader-Willi Syndrome), and taking weight loss Those who take medication or have undergone bariatric surgery. The 587 subjects who participated in the experiment were divided into the normal group (323 persons, BMI<27kg / m2) and the obese group (264 persons, BMI≧27kg / m2) according to the definition of adult obesity by the Taiwan Executive Yuan Department of Health. All subjects are required to agree to sign the human experiment consent form, and record their age, gender, height, weight, waist and hip circumference, body fat percentage, blood pressure, heartbeat and other data. The contents of the test were all reviewed and approved by the Mackay...

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Abstract

The invention provides a method of in-vitro measuring obesity-causing risk, which comprises following steps: (1) preparing a sample containing nucleic acid; (2) detecting the genotypes of a peroxisome proliferator-activated receptor (PPARG) allele and syndecan3 (SDC3) allele; and (3) identifying the genotypes of the PPARG allele and the SDC3 allele to obtain an identifying result, wherein if the PPARG allele and the SDC3 allele are mutated at the same time, the sample is sourced from an individual having increasing obesity-causing risk. The method is used for determining the obesity-causing risk with the genotypes of the PPARG gene and the SDC3 gene. Compared with a method in which the genotype of single gene is employed, the method is increased in the accuracy of prediction, so that possibility of mistake generated by determining the obesity-causing risk just by the same specific single gene in different individuals is reduced. The method also can be used as a basis for providing corresponding health-caring strategy.

Description

technical field [0001] The invention relates to a method for detecting the risk of obesity, especially a method for measuring the risk of obesity in vitro by using single nucleotide polymorphisms of multiple specific genes. Background technique [0002] Fat cells will exist in the form of pre-adipocytes at the beginning of the organism. When there is too much energy in the body, the pre-adipocytes will be prompted to differentiate into mature adipocytes and store fat in the fat cells Inside. When energy continues to be excessive, fat cells will continue to accumulate fat, causing fat cells to hypertrophy and developing into obesity. [0003] Peroxisome proliferator-activated receptors (PPARs) are a class of nuclear receptor proteins, mainly as transcription factors to regulate the expression of nuclear genes. Members of the PPARs gene family include PPARA, PPARB and PPARG, and PPARG has two isoforms: PPARG1 and PPARG2. PPARG1 exists in most tissues (except muscle tissue),...

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor蔡岳颖林咏翔关淑君黄雪茵
OwnerTCI GENE