Method for determining risk of obesity in vitro

An in-vivo and in-vitro detection technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of no significant correlation, inaccurate obesity risk, and different correlations, etc., and achieve risk improvement Effect

Active Publication Date: 2018-05-01
TCI GENE
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Problems solved by technology

However, the results of the European study found that the variation of SDC3 was not significantly related to the occurrence of obesity. Furthermore, the association between the SNP sites of each SDC3 and the occurrence of obesity was different.
[0005] Based on the above, it may still be controversial to use UCP3 or SDC3 gene alone as an obesity gene indicator. Specifically, there may still be inaccurate doubts about using UCP3 or SDC3 gene alone to detect the risk of obesity

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  • Method for determining risk of obesity in vitro
  • Method for determining risk of obesity in vitro
  • Method for determining risk of obesity in vitro

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Experimental program
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Embodiment

[0038] Materials and Methods

[0039]

[0040] This trial invites Taiwanese adults to participate through the Department of Family Medicine and Community Medicine Center of Mackay Memorial Hospital, and excludes those who are currently pregnant, cancer, secondary obesity, obesity caused by congenital chromosomal abnormalities (such as Prader-WilliSyndrome), and taking weight-loss drugs or those who have undergone bariatric surgery. The 587 subjects who participated in the experiment were divided into normal groups (323 persons, BMI2 ) and obesity group (264 people, BMI≧27kg / m 2 ). All subjects are required to agree to sign the human experiment consent form, and record their age, sex, height, weight, waist and hip circumference, body fat, blood pressure, pulse and other data. The contents of the test were all reviewed and approved by the Mackay Hospital Human Test Committee (IRB).

[0041]

[0042] Peripheral venous blood (about 3-5c.c) was collected from the subjects af...

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Abstract

The present invention provides a method for determining the risk of obesity in vitro, which includes the following steps: prepare a nucleic acid-containing sample, and detect uncoupling protein 3 (uncoupling protein 3, UCP3) and syndecan 3 ( syndecan3, SDC3) genotype of the allele; and, identify the genotype of the UCP3 and SDC3 allele of the sample, and obtain an identification result, the identification result is the simultaneous variation of the UCP3 and SDC3 allele, and the sample is derived from a Individuals at increased risk of obesity. The method uses polygenic genotypes to determine the risk of obesity, and compared with single-gene genotypes, the accuracy of prediction can be increased to reduce the risk of obesity in different individuals determined only by the same specific single gene. The possibility of error and can be used as a basis for providing corresponding health care countermeasures.

Description

technical field [0001] The present invention relates to a method for detecting the risk of obesity, especially a method for determining the risk of obesity in vitro by using single nucleotide polymorphisms of multiple specific genes Background technique [0002] The regulation of fat metabolism and thermogenesis affects the energy utilization efficiency of animals, while mitochondria are the positions involved in energy in animals, and their main function is to produce adenosine triphosphate (ATP) as an energy source for cells to use. The production of ATP is obtained through the phosphorylation coupling reaction of adenosine diphosphate (ADP) by ATP synthase on the inner membrane. The so-called coupling reaction (coupling) refers to a kind of chemoosmotic effect of mitochondria binding phosphorylation reaction and proton flow (proton flow). ATP production can be regulated by uncoupling protein (UCP), so that energy is not stored in the form of ATP, but released in the form...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12Q1/6883C12N15/11
Inventor蔡岳颖林咏翔关淑君黄雪茵
OwnerTCI GENE