Method for determining risk of obesity in vitro

A technology of external detection and receptors, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of no significant correlation, different correlations, inaccuracy, etc., and achieve the goal of obesity Risk-enhancing effects

Active Publication Date: 2017-12-05
TCI GENE
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Problems solved by technology

However, the results of the European study found that the variation of SDC3 was not significantly related to the occurrence of obesity. Furthermore, the association between the SNP sites of each SDC3 and the occurrence of obesity was different.
[0006] Based on the above, it may still be controversial to use PPARG or SDC3 polymorphism alone as an obesity genetic indicator. Specifically, it may still be inaccurate to use PPARG or SDC3 polymorphism alone to detect the risk of obesity

Method used

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  • Method for determining risk of obesity in vitro
  • Method for determining risk of obesity in vitro
  • Method for determining risk of obesity in vitro

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Experimental program
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Embodiment

[0045] Materials and Methods

[0046]

[0047] This trial invites Taiwanese adults to participate through the Department of Family Medicine and Community Medicine Center of Mackay Memorial Hospital, and excludes those who are currently pregnant, cancer, secondary obesity, obesity caused by congenital chromosomal abnormalities (such as Prader-WilliSyndrome), and taking weight-loss drugs or those who have undergone bariatric surgery. The 587 subjects who participated in the experiment were divided into the normal group (323 persons, BMI<27kg / m2) and the obese group (264 persons, BMI≧27kg / m2) according to the definition of adult obesity by the Taiwan Executive Yuan Department of Health. All subjects are required to agree to sign the human experiment consent form, and record their age, gender, height, weight, waist and hip circumference, body fat percentage, blood pressure, heartbeat and other data. The contents of the test were all reviewed and approved by the Mackay Hospital ...

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Abstract

The present invention provides a method for determining the risk of causing obesity in vitro, comprising the following steps: prepare a nucleic acid-containing sample, and detect the peroxisome proliferator-activated receptor (PPARG) and polyglobulin in the sample. the genotype of the allele of proteoglycan 3 (syndecan3, SDC3); The sample is from an individual at increased risk of obesity. The method is to use the genotypes of PPARG and SDC3 genes to determine the risk of obesity, and compared with the genotype of a single gene, the accuracy of prediction can be increased to reduce the risk of obesity caused by different individuals only determined by the same specific single gene. Obesity risk yields the possibility of error and can be used as a basis for providing corresponding health care strategies.

Description

technical field [0001] The invention relates to a method for detecting the risk of obesity, especially a method for measuring the risk of obesity in vitro by using single nucleotide polymorphisms of multiple specific genes. Background technique [0002] Fat cells will exist in the form of pre-adipocytes at the beginning of the organism. When there is too much energy in the body, the pre-adipocytes will be prompted to differentiate into mature adipocytes and store fat in the fat cells Inside. When energy continues to be excessive, fat cells will continue to accumulate fat, causing fat cells to hypertrophy and developing into obesity. [0003] Peroxisome proliferator-activated receptors (PPARs) are a class of nuclear receptor proteins, mainly as transcription factors to regulate the expression of nuclear genes. Members of the PPARs gene family include PPARA, PPARB and PPARG, and PPARG has two isoforms: PPARG1 and PPARG2. PPARG1 exists in most tissues (except muscle tissue),...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor蔡岳颖林咏翔关淑君黄雪茵
OwnerTCI GENE