Method for determining risk of obesity in vitro

An in-vivo and in-vitro detection technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of no significant correlation, different correlations, inaccurate obesity risk, etc., and achieve obesity The risk-enhancing effect of

Active Publication Date: 2018-05-25
TCI GENE
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Problems solved by technology

However, the results of the European study found that the variation of SDC3 was not significantly related to the occurrence of obesity. Furthermore, the association between the SNP sites of each SDC3 and the occurrence of obesity was different.
[0006] Based on the above, it may still be controversial to use the ESR1 or SDC3 gene alone as an obesity gene indicator. Specifically, there may still be inaccurate doubts about using the ESR1 or SDC3 gene alone to detect the risk of obesity

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  • Method for determining risk of obesity in vitro
  • Method for determining risk of obesity in vitro
  • Method for determining risk of obesity in vitro

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Embodiment

[0042] Materials and Methods

[0043]

[0044] This trial invites Taiwanese adults to participate through the Department of Family Medicine and Community Medicine Center of Mackay Memorial Hospital, and excludes those who are currently pregnant, cancer, secondary obesity, obesity caused by congenital chromosomal abnormalities (such as Prader-WilliSyndrome), and taking weight-loss drugs or those who have undergone bariatric surgery. The 587 subjects who participated in the experiment were divided into normal groups (323 persons, BMI2 ) and obesity group (264 people, BMI≧27kg / m 2 ). All subjects are required to agree to sign the human experiment consent form, and record their age, gender, height, weight, waist and hip circumference, body fat percentage, blood pressure, heartbeat and other data. The contents of the test were all reviewed and approved by the Mackay Hospital Human Test Committee (IRB).

[0045]

[0046] Peripheral venous blood (about 3-5c.c) was collected fr...

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Abstract

The invention provides a method of in-vitro measuring obesity-causing risk, which comprises following steps: (1) preparing a sample containing nucleic acid; (2) detecting the genotypes of an estrogen receptor 1 (ESR1) allele and a syndecan3 (SDC3) allele; and (3) identifying the genotypes of the ESR1 allele and the SDC3 allele to obtain an identifying result, wherein if the ESR1 allele and the SDC3 allele are mutated at the same time, the sample is sourced from an individual having increasing obesity-causing risk. The method is used for determining the obesity-causing risk with the genotypes of the ESR1 gene and the SDC3 gene. Compared with a method in which the genotype of single gene is employed, the method is increased in the accuracy of prediction, so that possibility of mistake generated by determining the obesity-causing risk just by the same specific single gene in different individuals is reduced. The method also can be used as a basis for providing corresponding health-caring strategy.

Description

technical field [0001] The invention relates to a method for detecting the risk of obesity, especially a method for measuring the risk of obesity in vitro by using single nucleotide polymorphisms of multiple specific genes. Background technique [0002] Estrogen receptor (ESR) mainly has two forms, ESR1 and ESR2, which are expressed in most tissues of the human body, but the expression level will vary according to the tissue, biological age and female menstrual period. The main functions of ESR can be divided into two categories, one is as a cell membrane receptor, which can combine with estrogen to start the second message (secondmassager) system to regulate human physiological responses; the other function can be as a transcription factor in the nucleus, through direct or indirect binding Regulate downstream gene expression. [0003] In animal experiments, it was found that male and female mice with deletion of estrogen receptor α (ERα, same as ESR1 gene) had significantl...

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12Q1/6813C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor蔡岳颖林咏翔关淑君黄雪茵
OwnerTCI GENE