Preparation method of FTO demethylase detection kit and detection kit

A technology of demethylase and detection kit, which is applied in the field of clinical medicine and biological products, can solve the problems such as the lack of immunological detection methods, and achieve the effects of fast detection speed, high yield and improved detection sensitivity

Pending Publication Date: 2022-03-01
余朴芬
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of FTO demethylase mainly focuses on genotyping or PCR methods at the molecular level, and there is no rapid immunoassay method

Method used

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Examples

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Embodiment Construction

[0024] Exemplary embodiments of the present invention are described in detail below in conjunction with practical applications. In the following detailed description, for the convenience of explanation, many specific details are set forth to provide a comprehensive understanding of the embodiments of the present disclosure. The following examples are only used to illustrate the present invention and should not be considered as limiting the scope of the present invention. It may be evident, however, that one or more embodiments may be practiced without these specific details. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer.

[0025] The experimental methods used in the following examples are conventional methods unless otherwise specified, and the materials and reagents used can be obtained from commercial sources unless otherwise specified. Among them, th...

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Abstract

The invention discloses a preparation method of an FTO demethylase detection kit, the detection kit and application. The preparation method comprises the following steps: preparing a sample pad: soaking a nitrocellulose membrane in a phosphate buffer solution containing BSA (Bovine Serum Albumin) with the mass concentration of 2%, 0.1 M NaCl and a surfactant at 4 DEG C for 8 hours, and drying to obtain the sample pad; treating the filtering membrane; preparing a combination pad: respectively labeling fluorescent microspheres on antibodies containing four sites of FTO demethylase, adding a microsphere diluent, and uniformly mixing to obtain an antibody mixed solution; uniformly laying the antibody mixed solution on a treated sample pad, carrying out vacuum freeze drying, and sealing to obtain a conjugate pad; preparing a chromatographic membrane: respectively preparing a detection band and a quality control band on the nitrocellulose membrane to obtain the chromatographic membrane; preparing the absorbent paper; and assembling. The FTO demethylase kit disclosed by the invention is helpful for obese high-risk persons to know own genetic conditions in advance, and obesity and overweight are avoided by adjusting dietary structures and lifestyles in advance.

Description

technical field [0001] The present invention relates to the technical fields of clinical medicine and biological products, in particular to a preparation method of a FTO demethylase detection kit and a FTO demethylase detection kit, in particular to a method for identifying the risk of obesity A preparation method and a detection kit for a demethylase detection kit. Background technique [0002] Obesity is not only related to external lifestyle, but also related to individual genetic factors, that is, obesity genes. Obesity genes include fat mass and obesity associated (FTO), leptin (LEP) and other genes. Among them, the FTO gene is located on the 16q12.2 chromosome, with a total length of about 430kb. It encodes a demethylase and participates in diet regulation, energy balance, fat metabolism and transcription regulation. FTO is currently recognized as having the strongest correlation with obesity. most exact gene. FTO protein is mainly expressed in fat, pancreas, liver,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/573G01N33/533G01N33/543G01N33/558G01N33/68
CPCC12Q1/6883G01N33/573G01N33/558G01N33/533G01N33/54313G01N33/6893C12Q2600/156G01N2333/90251G01N2800/044
Inventor 不公告发明人
Owner 余朴芬
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