Preparation and detection method of total flavonoids of Melaleuca alternifolia

A technology of Melaleuca alternifolia and total flavonoids is applied in the field of preparation of flavonoid extracts, which can solve the problems of wasting medicinal material resources and failing to fully develop and utilize plants, and achieves good application prospects, reasonable design and short production cycle. Effect

Active Publication Date: 2018-05-18
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

) The branches and leaves of Melaleuca alternifolia are extracted by water distillation to extract essential oils, which are usually treated as production waste. The plant parts after oil extraction are discarded as waste, and the plants have not been fully developed and utilized, seriously wasting medicinal resources

Method used

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  • Preparation and detection method of total flavonoids of Melaleuca alternifolia
  • Preparation and detection method of total flavonoids of Melaleuca alternifolia
  • Preparation and detection method of total flavonoids of Melaleuca alternifolia

Examples

Experimental program
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Effect test

Embodiment 1

[0031]Take 1000g of Melaleuca alternifolia branches and leaves, add 3L of water, heat for 70 minutes, extract the volatile oil, after the oil is collected, discard the aqueous solution in the container, add 12L of 75% ethanol, heat and reflux to extract 3 times, each time for 2 hours, and combine the extracts , filtered, and the filtrate was recovered under reduced pressure until it had no ethanol smell, dissolved in water, and made into an extract of Melaleuca alternifolia with a total flavonoid concentration of 0.5 mg / mL. Take 80 g of the processed X-5 macroporous adsorption resin (equivalent to 30 g of dry resin, column volume 200 mL), and wet-pack the column, and put 600 mL of Melaleuca alternifolia extract at 3 BV / h Adsorb at the column flow rate, elute with 3 BV of water until the eluate is basically clear, discard the water eluate, elute with 4BV 75% ethanol at a flow rate of 2BV / h, collect the eluate, and recover ethanol under reduced pressure. 5%。 Concentrated to dryn...

Embodiment 2

[0033] Take 1200g of Melaleuca alternifolia branches and leaves, add 4L of water, heat for 70 minutes, extract the volatile oil, after the oil is collected, discard the aqueous solution in the container, add 15L of 50% ethanol, heat and reflux for extraction 3 times, each time for 3 hours, and combine the extracts , filtered, and the filtrate was recovered under reduced pressure until it had no ethanol smell, dissolved in water, and made into an extract of Melaleuca alternifolia with a total flavonoid concentration of 0.8 mg / mL. Get 60g of X-5 macroporous adsorption resin that has been processed (equivalent to 20g of dry resin, column volume 150mL), wet packing column, 750mL Melaleuca alternifolia extract is adsorbed with the upper column flow rate of 2BV / h, Elute with 4BV of water until the eluate is basically clear, discard the water eluate, elute with 5BV of 50% ethanol at a flow rate of 4BV / h, collect the eluate, recover ethanol under reduced pressure, concentrate to drynes...

Embodiment 3

[0035] Take 800g of Melaleuca alternifolia branches and leaves, add 2L of water, heat for 70 minutes, extract the volatile oil, after the oil is collected, discard the aqueous solution in the container, add 8L of 95% ethanol, heat and reflux for extraction 3 times, each time for 1 hour, and combine the extracts , filtered, and the filtrate was recovered under reduced pressure until it had no ethanol smell, dissolved in water, and made into an extract of Melaleuca alternifolia with a total flavonoid concentration of 0.2 mg / mL. Take 50g of the treated AB-8 macroporous adsorption resin (equivalent to 20g of dry resin, column volume 150mL), wet-pack the column, and absorb 450mL of Melaleuca alternifolia extract at a column flow rate of 0.5BV / h , eluted with 4BV of water until the eluate was basically clear, discarded the water eluate, eluted with 3BV of 80% ethanol at a flow rate of 5BV / h, collected the eluate, recovered ethanol under reduced pressure, concentrated to dryness, 60 ...

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Abstract

The invention discloses an extract of total flavonoids of Melaleuca alternifolia and a preparation method thereof. The essential oil is extracted from the branches and leaves of Melaleuca alternifolia after extracting essential oils with water vapor, heated and refluxed with ethanol, the ethanol is recovered under reduced pressure and concentrated to no alcohol smell. It is prepared by separation, purification and drying with macroporous adsorption resin, and the content of total flavonoids of Melaleuca alternifolia is determined by UV-visible spectrophotometry. The method is accurate, stable and easy to operate, which can meet the needs of industrial production and can effectively control Quality of total flavonoids extract of Melaleuca alternifolia. The total flavonoid content of Melaleuca alternifolia total flavonoids extract of the present invention can reach more than 50%, has low toxicity, has anti-inflammatory, antibacterial, analgesic, anti-allergic, anti-oxidation and other pharmacological effects, and the preparation method is reasonable, stable, and the production cycle is short. Short and suitable for industrial production, it is of great significance to the deep development and utilization of Melaleuca alternifolia resources and has a good development prospect.

Description

technical field [0001] The invention relates to the field of preparation of flavonoid extracts, in particular to a purification preparation and detection method of total flavonoids of Melaleuca alternifolia. Background technique [0002] Natural plant extracts use plants as raw materials, according to the purpose and needs of the extraction, use physical and chemical extraction and separation methods to obtain and concentrate one or more active ingredients in the plant without changing the structure of the active ingredients. formed products. Compared with traditional Chinese herbal medicines and proprietary Chinese medicines, Chinese herbal extracts have clear active ingredients, quantitative indicators for quality control, and most of the harmful ingredients or impurities are removed. They are more safe, effective, and controllable than traditional herbal medicines. get promoted. In addition, traditional Chinese medicine extracts also incorporate new modern pharmaceutica...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/61A61P29/00A61P31/04A61P37/08A61P39/06G01N21/31A61K127/00A61K135/00
Inventor 刘布鸣林霄邱宏聪柴玲黄艳
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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