Application of angelica vine in preparation of medicine for treating Alzheimer's disease
A technology of angelica vine, applied in the direction of medical formula, drug combination, plant raw materials, etc., to achieve the effect of high safety, prolonging the latent period of avoiding darkness, and protecting the brain from damage
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Embodiment 1
[0032] Embodiment 1: the preparation of angelica vine extract
[0033] Take 10kg Angelica sinensis and pulverize it, add 80% ethanol water bath to extract 3 times, the amount of ethanol each time is 12 times, 10 times, 8 times of the total weight of medicinal materials, the water bath temperature is 70 ℃, and the extraction time is respectively 60min, 50min, 40min, combine the extracts, concentrate, filter, and dry to obtain Angelica vine extract.
[0034] Taking rutin as the reference substance, the yield of total flavonoids from Angelica vine was 0.35%.
Embodiment 2
[0035] Embodiment 2: the preparation of the total flavonoids of Angelica vine
[0036]Take 10kg Angelica vine and pulverize it, add 80% ethanol, extract 2-3 times with 780W microwave, each ethanol dosage is 12 times of the total weight of medicinal materials, each extraction time is 15min, combine the extracts, concentrate under reduced pressure, Add diatomaceous earth to filter, and the filtrate is adsorbed by ADS17 resin, eluted with 70% ethanol, the eluate is concentrated, and vacuum-dried to obtain total flavonoids of Angelica vine.
[0037] Taking rutin as the reference substance, the yield of total flavonoids from Angelica vine was 0.49%.
Embodiment 3
[0038] Embodiment 3: the preparation of the total flavonoids of Angelica vine
[0039] Take 10kg of Angelica sinensis and grind it, add 60% ethanol and microwave extraction 3 times, the amount of ethanol each time is 15 times, 10 times, 8 times of the total weight of the medicinal materials, and the extraction time is 20min, 15min, 10min respectively, combined The extract is concentrated and filtered, the filtrate is adsorbed by ADS17 resin, eluted with 80% ethanol, the eluate is concentrated, and vacuum-dried to obtain the total flavonoids of Angelica vine.
[0040] Taking rutin as the reference substance, the yield of total flavonoids in Angelica vine was 0.66%.
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