Preparation method of bitter gourd polypeptide and application thereof

A technology of bitter melon polypeptide and bitter melon, which is applied in the field of preparation of bitter melon polypeptide, and can solve problems such as not being able to meet the requirements of bitter melon polypeptide

Pending Publication Date: 2020-10-30
SHAANXI HUIKE BOTANICAL DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the previous bitter gourd polypeptides largely failed to meet the market's requirements for bitter gourd polypeptides.

Method used

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  • Preparation method of bitter gourd polypeptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, see figure 1 , a preparation method of bitter gourd polypeptide, comprising the following steps:

[0029] 1) After crushing the bitter gourd raw material into small particles of 10-60 mesh, add it into water according to the ratio of 1:1 to 10, heat the broken bitter gourd raw material in a water bath, adjust the pH to alkaline, and control the extraction temperature at 45- At about 60°C, the extraction time is about 1.5-3h, and the bitter gourd protein solution is obtained by filtration; wherein, the bitter gourd protein solution in step 1) is the bitter gourd protein solution after the bitter gourd raw material is heated and extracted in a water bath, and the pH of the solution is adjusted to precipitate the protein .

[0030] 2) Adjust the pH value of the bitter gourd protein solution obtained in step 1) to 3-5 to precipitate the protein, wash the precipitate with water to neutrality, then add the compound enzyme solution to enzymolyze the bitter gourd pr...

Embodiment 2

[0038] Example 2, see figure 1 , a preparation method of bitter gourd polypeptide, comprising the following steps:

[0039] 1) After crushing the bitter gourd raw material into 10-mesh small particles, add it into water at a ratio of 1:5, heat the crushed bitter gourd raw material in a water bath, adjust the pH to alkaline, control the extraction temperature at about 50°C, and extract the time For about 2 hours, the bitter gourd protein solution is obtained by filtration; wherein, the bitter gourd protein solution in step 1) is the bitter gourd protein solution after the bitter gourd raw material is heated and extracted in a water bath, the pH of the solution is adjusted, and the protein is precipitated.

[0040] 2) Adjust the pH value of the bitter gourd protein liquid obtained by filtering in step 1 to 4, precipitate the protein, wash the precipitate with water to neutrality, and then add a compound enzyme solution to enzymolyze the bitter gourd protein. The enzymatic hydrol...

Embodiment 3

[0048] Example 3, see figure 1 , a preparation method of bitter gourd polypeptide, comprising the following steps:

[0049] 1) After crushing the bitter gourd raw material into small particles of 35 mesh, add it into water at a ratio of 1:1, heat the crushed bitter gourd raw material in a water bath, adjust the pH to alkaline, control the extraction temperature at about 45°C, and extract the time is about 1.5, and the bitter gourd protein solution is obtained by filtration; wherein, the bitter gourd protein solution in step 1) is the bitter gourd protein solution after the bitter gourd raw material is heated and extracted in a water bath, the pH of the solution is adjusted, and the protein is precipitated.

[0050] 2) Adjust the pH value of the bitter gourd protein liquid obtained by filtering in step 1 to 3, precipitate the protein, wash the precipitate with water to neutrality, and then add the compound enzyme solution to enzymolyze the bitter gourd protein. The enzymatic hy...

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Abstract

The invention relates to a preparation method of bitter gourd polypeptide and an application thereof. The preparation method comprises the following steps: 1) crushing a bitter gourd raw material, adding the crushed bitter gourd raw material into water according to a ratio of 1: (1-10), adjusting, and filtering to obtain a bitter gourd protein solution; (2) adjusting the bitter gourd protein liquid filtered in the step (1) until the bitter gourd protein liquid is washed and precipitated to be neutral, adding a compound enzyme, carrying out enzymolysis on bitter gourd protein, carrying out inactivation treatment on the compound enzyme after the enzymolysis is finished, then naturally cooling the inactivated bitter gourd protein liquid to room temperature, and removing insoluble substances and precipitation impurities to obtain clarified bitter gourd crude polypeptide subjected to enzymolysis; and 3) screening the molecular weight of the bitter gourd crude polypeptide subjected to enzymolysis by adopting a membrane filtration technology to obtain bitter gourd polypeptide with the molecular weight of 3kDa or below, concentrating the volume, and drying to obtain the bitter gourd polypeptide powder. The content of effective polypeptide molecules is increased, and the taste of bitter gourd polypeptide is improved. The product can be used in the fields of preparation of special diet,health food, medicines and the like with the function of regulating blood glucose metabolism. The product is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of bioactive peptide preparation, and relates to a preparation method and application of bitter gourd polypeptide. Background technique [0002] With the continuous improvement of people's living standards and the rapid development of social economy, the number of diabetic patients in my country has shown a rapid growth trend, accounting for about one-third of the total number of diabetic patients in the world. At present, the incidence of diabetes in my country is as high as 9.6%, and the number of diabetic patients has exceeded 110 million, and the number of patients is still increasing year by year. The harm of diabetes is huge, and the complications of diabetes accompanied by diabetes also pose a huge threat to the health and life of patients. Diabetes is called diabetes in ancient times, and it refers to a syndrome characterized by polydipsia, polyuria, polyphagia, weight loss, fatigue, and sweet urine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/36C07K1/34C07K1/30A23L33/18A61K38/01A61P3/10
CPCC12P21/06C07K1/14C07K1/36C07K1/34C07K1/30A23L33/18A61K38/011A61P3/10A23V2002/00A23V2200/328
Inventor 陈冠宇王佳明王蕾阮娜
Owner SHAANXI HUIKE BOTANICAL DEV CO LTD
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