Method for long-term intracerebral pump injection of drugs in great quantity used for primate

Abstract

The invention discloses a method for long-term intracerebral pump injection of drugs in the great quantity used for a primate. The method comprises following steps: step1, positioning and opening a skull of an animal-determining an injection position and drilling the animal skull after general anesthesia is carried out to the animal; step2, imbedding a silica gel syringe needle into a brain; step3, pulling out a hard metal needle core of a silica gel syringe needle; step 4, connecting with a micro-pressure pump and starting intracerebral injection of a drug solution or an experiment reagent; step5, pulling out the silica gel syringe needle after finishing injection. In the step 5, the silica gel syringe needle is not pulled out in need of multiple dosing on a long-term basis. The micro-pressure pump is embedded into the skull surface under the head skin and an injection syringe is utilized for puncturing the scalp and injecting drugs into the micro-pressure pump for multiple drug injection on a long-term basis. The method for long-term intracerebral pump injection of drugs in the great quantity used for the primate has following beneficial effects: technology of pump injection of drugs is safe and reliable so as to effectively avoid intracerebral mass effect and effectively avoid countercurrent flow of drugs in case of multiple drug injection; intracerebral dosage is carried out for multiple times; and skull-opening operation is avoided for injection at each time so as to greatly simplify procedures for experimental operation, thereby improving survival quality of an experimental animal and scientific credibility of experimental data.

Description

technical field [0001] The invention relates to a drug pumping technology, in particular to a method for pumping a large amount of drugs in the primate brain for a long time. Background technique [0002] In the study of primate central nervous system diseases, when it is necessary to inject research drugs such as cells or neurotrophic factors into the brain, it is usually carried out using a microinjector under the guidance of MRI positioning, and no additional pressure can be applied during injection. Microinjector And the injection needle is rigid, and the injection needle has no core. Depending on the injection target, each injection volume can reach up to 5-20 microliters. [0003] There are the following two disadvantages when using a micro-injector for intracerebral injection: 1. The amount of each injection is only about 20 microliters at most. The needle tract backflows out of the brain, causing secondary damage or causing insufficient dosage; 2. When using a micro...

AI Technical Summary

Problems solved by technology

[0003] There are the following two disadvantages when using a micro-injector for intracerebral injection: 1. The amount of each injection is only about 20 microliters at most. The needle tract backflows out of the brain, causing secondary damage or causing insufficient dosage; 2. When using a micro-syringe for intracerebral injection, do not continue to administer the drug for a long time, and each injection requires a craniotomy, which is cumbersome and risky. bigger