DC cell-based HPV virus vaccine preparation method

An HPV virus and cell technology, applied in the field of HPV virus vaccine and its preparation, can solve the problems of long time required for culturing cells, prolonged treatment period of patients and high cost

Inactive Publication Date: 2015-10-28
SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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Problems solved by technology

The target HPV antigen-specific CTL cells prepared by this method are the subdivision of white blood cells, and are a kind of biological treatment technology for the treatment of HPV infection, but the cost of this method is high, and the time required for culturing the cells is long, which leads to the treatment cycle of the patient. extend

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  • DC cell-based HPV virus vaccine preparation method
  • DC cell-based HPV virus vaccine preparation method
  • DC cell-based HPV virus vaccine preparation method

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Embodiment Construction

[0022] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only the present invention. Some embodiments of the invention are not all of the embodiments.

[0023] The invention provides a preparation method of an HPV virus vaccine based on DC cells. DC cells (dendritic cells) are professional APCs (antigen-presenting cells) with the strongest function of presenting antigens. They can effectively ingest and process antigens, activate initial T cells, and play a key role in the induction of immune responses. The invention utilizes the strong antigen presenting ability of DC cells to load the HPV-related antigens, which can effectively break immune tolerance, restore cellular immune response and eliminate...

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Abstract

The invention provides a DC cell-based HPV virus vaccine preparation method. The method comprises the following steps: separating CD14+ cells from blood to obtain the CD14+ cells; inducing the CD14+ cells in a medium to obtain immature DC cells; adding HPV16 chimeric virus-like particles to the medium to promote proliferation of the immature DC cells, and co-culturing the HPV16 chimeric virus-like particles and the immature DC cells for a period of time to carry out antigen loading; and promoting maturation of antigen supported immature DC cells. The CD80 expression level and the CD83 expression level of the DC cells generated after culture are obviously improved, and the IL-12 secretion ability of the DC cells is also greatly increased. More importantly, the CD80 expression level and the CD83 expression level of a DC cell vaccine prepared through the preparation method provided by the invention are higher than those of DC cell vaccines in the prior art.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an HPV virus vaccine and a preparation method thereof. Background technique [0002] On the market, there are HPV compound multivalent epitope peptide vaccines, which are artificially synthesized by linking different antigenic epitopes together or linking multiple antigenic peptides on a branched oligolysine framework to form a three-dimensional structure A complex multivalent epitope peptide vaccine loaded with a variety of selected epitope peptides was constructed. This method can effectively enhance the immunogenicity of polypeptides, but the cost of polypeptide synthesis is high, especially the synthesis of complex polypeptides with three-dimensional structures is difficult; and it is difficult to maintain a stable three-dimensional structure. At the same time, studies have confirmed that peptide vaccines have strong specificity but The immunogenicity is weak, and it cannot trigge...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/29A61P31/14C12N5/0784
Inventor 曾宪卓鲁菲
Owner SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
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