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A method for cultivating Schizochytrium high-yield DHA

A high-yield Schizochytrium technology, applied in the field of microbial fermentation, can solve the problems of high equipment investment and maintenance costs, high production costs, and low efficiency, and achieve the effects of reducing fermentation risks, high production levels, and increasing profits

Active Publication Date: 2019-02-12
QINGDAO XUNON BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional industrial production of algal oil DHA mainly uses glucose as a carbon source, but the production of 1 ton of DHA oil requires about 5 tons of glucose. This conversion is inefficient. In addition, a large amount of energy needs to be input during the production process, and equipment investment and maintenance costs are expensive. , making the current production cost of algal oil DHA very high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Prepare culture medium

[0031] The preparation method of the shake flask culture medium is as follows: after weighing the components in the formula, manganese chloride tetrahydrate, zinc sulfate heptahydrate, copper sulfate pentahydrate, sodium molybdate dihydrate, and cobalt chloride hexahydrate, use sterile water Fully dissolve to prepare a mother liquor, under acidic conditions, sterilize under high-pressure steam at 121°C for 20 minutes; after weighing other components, fully dissolve them with sterile water, dispense them into shake flasks, and sterilize under high-pressure steam at 121°C for 20 minutes.

[0032] Shake flask medium formula is as follows: glucose 45g / L, yeast extract 10g / L, sodium sulfate 8.0g / L, potassium chloride 0.7g / L, magnesium sulfate 2.0g / L, potassium dihydrogen phosphate 2.0g / L , calcium chloride 0.15g / L, ammonium sulfate 1.0g / L, manganese chloride tetrahydrate 1.7mg / L, zinc sulfate heptahydrate 3.0mg / L, copper sulfate pentahydrate 1.5m...

Embodiment 2

[0041] Compared with Example 1, except that the method of fermenting and cultivating in a 3000L fermenter is different, all the others are the same, specifically: 150L of the seed liquid in the primary seed tank of Example 1 is all inoculated into a 1500L fermenter for cultivation. Fermentation culture is carried out in a 3000L fermenter based on the base, the culture temperature is 28 ℃, and the ventilation rate is 0.5vvm. The whole fermentation process utilizes 30% ammonia water to control the pH of the fermented liquid between 5.5~6.0. When fermented to 20h, start to feed 750g / L glucose solution and 230g / L mixed nitrogen source solution (this solution contains 30g / L yeast extract, 100g / L sodium glutamate, 100g / L corn steep liquor dry powder) , Sampling every 3 hours to detect the glucose and total nitrogen content in the fermentation broth, the glucose concentration in the fermentation broth is always maintained at 30-50g / L, and the total nitrogen content is maintained at 15...

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Abstract

The invention discloses a method for cultivating high-yield DHA (docosahexaenoic acid) through schizochytrium, and belongs to the field of microbial fermentation. The method comprises steps as follows: a schizochytrium seed solution with the dry cell weight being 18-22 g / L after culturing in a shake flask is inoculated into a seed tank and then inoculated into a fermentation tank after being cultured to reach an inoculation transfer level, high-density culture is performed at the high temperature being 22 DEG C-33 DEG C in an early stage, induction culture is performed in a threatening manner for 110-130 hours at the low temperature being 18 DEG C-22 DEG C in a late stage, and glucose and total nitrogen are kept in a certain concentration and pH (potential of hydrogen) is kept ranging from 5.5 to 6.6 through feeding of a glucose solution, a nitrogen source solution and an alkaline solution in the fermentation process. The dry cell weight of a fermentation solution obtained with the method reaches 120g / L-150 g / L, the density of the DHA reaches 40g / L-50 g / L and is 1.2-1.5 times higher than reported DHA productivity, and the method is very suitable for industrial production of DHA.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and relates to the production of DHA, in particular to a method for cultivating Schizochytrium high-yielding DHA. Background technique [0002] Docosahexaenoic acid (22:6n-3, docosahexaenoic acid, DHA) is an important omega-3 series polyunsaturated fatty acids (omega-3 polyunsaturated fatty acids), which can not only promote the intelligence and vision development of infants and young children , It also has the effects of preventing cardiovascular diseases in the elderly, anti-cancer, anti-inflammatory, and enhancing immunity. The traditional method of extracting polyunsaturated fatty acids is to obtain them from deep-sea fish oil. With the environmental pollution and the gradual scarcity of marine fishery resources, there are many problems in the extraction of unsaturated fatty acids from deep-sea fish, such as unstable quality and exogenous pollution. , bad smell, high purification cost, e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64
Inventor 徐瑶王志臻郑紫薇李慧玲王冰峰曹腾腾
Owner QINGDAO XUNON BIOLOGICAL ENG
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