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Preparing method for medium-concentration IAA gel

A preparation method and gel technology, applied in the field of plant biology, can solve the problems of branch loss, high cost, and many technical steps, and achieve the effect of reducing water evaporation and easy operation

Inactive Publication Date: 2015-12-02
钱夕刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment

[0011] A method for preparing medium-concentration IAA gel, characterized in that the method includes preparation of hormone mother solution, preparation of working solution, preparation of hormone gel, and the specific operation steps are as follows:

[0012] ① Hormone mother solution preparation: Accurately weigh 0.1g of IAA and dissolve it with a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL IAA mother solution;

[0013] ②Preparation of working solution: dilute the above mother solution according to the volume ratio of 1:10 into a medium-concentration hormone working solution with a concentration of 100mg / L;

[0014] ③ Hormone gel preparation: Accurately weigh 1 g of sodium carboxymethylcellulose and 8 g of sodium alginate, and dissolve them in 100 mL of hormone working solution with a concentration of 100 mg / L in a water bath at 50 °C for later use.

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Abstract

The invention discloses a preparing method for medium-concentration IAA gel and belongs to the technical field of plant biotechnology. The preparing method comprises the steps that firstly, an IAA mother solution with the concentration of 1 mg / mL is prepared; secondly, the mother solution is diluted into an IAA working solution with the medium concentration of 100 mg / L; thirdly, the working solution is used for preparing medium-concentration IAA gel confining liquid containing sodium carboxymethyl cellulose with the mass concentration of 1% and sodium alginate tech grade with the mass concentration of 8%.

Description

technical field [0001] The invention discloses a method for preparing medium-concentration IAA gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as IAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propagation or roo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/38A01P21/00A01G1/00A01G7/06
Inventor 钱夕刚
Owner 钱夕刚