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Preparing method of medium-concentration IAA and glycerogel

A preparation method, glycerin gel technology, applied in the field of plant biology, can solve the problems of high cost, loss of branches, unsatisfactory production effect, etc., and achieve the effect of easy operation and reduced water evaporation

Inactive Publication Date: 2015-10-28
钱夕刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment

[0011] A method for preparing medium-concentration IAA and glycerin gel, characterized in that the method includes hormone mother solution preparation, working solution preparation, and hormone gel preparation, and the specific steps are as follows:

[0012] ① Hormone mother solution preparation: Accurately weigh 0.1g of IAA and dissolve it in a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL IAA mother solution;

[0013] ② Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:10 into a medium-concentration hormone working solution with a concentration of 100mg / L, and add 10ml of glycerin to 90ml of hormone working solution;

[0014] ③ Hormone gel preparation: Accurately weigh 1 g of sodium carboxymethylcellulose and 8 g of sodium alginate, and dissolve them in 100 mL of hormone and glycerol working solution with a concentration of 100 mg / L in a water bath at 50°C for later use.

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Abstract

The invention discloses a preparing method of medium-concentration IAA and glycerogel, and belongs to the technical field of plant biology. According to the method, firstly, 1mg / mL IAA mother liquid is prepared; the mother liquid is diluted into 100mg / L medium-concentration IAA and glycerinum work liquid; the work liquid is used for preparing medium-concentration IAA and glycerogel sealing liquid containing sodium carboxymethylcellulose with the mass concentration being 1 percent and sodium alginate with the mass concentration being 8 percent.

Description

technical field [0001] The invention discloses a method for preparing medium-concentration IAA and glycerin gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as IAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/38A01P21/00A01G7/06A01G1/00
Inventor 钱夕刚
Owner 钱夕刚