Node position use method of low-concentration 2,4-D and glycerogel

Abstract

The invention discloses a node position use method of low-concentration 2,4-D and glycerogel, and belongs to the technical field of plant biology. The method comprises the steps that a 1 mg / mL 2,4-D mother solution is prepared; the mother solution is diluted into a 10 mg / L low-concentration 2,4-D working solution; low-concentration 2,4-D and glycerogel confining liquid with the mass concentration of sodium carboxymethyl cellulose equal to 1% and the mass concentration of sodium alginate equal to 8% is prepared through the working solution; branches are inserted into a culture medium; the gel confining liquid is dropwise added on axillary buds of the healthy and thick branches.

Description

technical field [0001] The invention discloses a method for using low-concentration 2,4-D and glycerin gel at nodal sites, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as 2,4-D, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in...

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients

However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.