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Root dipping method of medium-concentration KT gel

A KT gel and concentration technology, applied in the field of plant biology, can solve the problems of branch loss, high cost, unsatisfactory production effect, etc., and achieve the effect of reducing water evaporation and easy operation.

Inactive Publication Date: 2015-11-18
何其家
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment

[0013] A method for dipping roots with medium-concentration KT gel, characterized in that the method includes hormone mother liquor preparation, working solution preparation, hormone gel preparation, root dipping, and cuttings, and the specific steps are as follows:

[0014] ① Hormone mother liquor preparation: Accurately weigh 0.1g of KT and dissolve it with a little 1M KOH, then dilute to 100mL with distilled water, which is 1mg / mL KT mother liquor;

[0015] ②Preparation of working solution: dilute the above mother solution according to the volume ratio of 1:10 into a medium-concentration hormone working solution with a concentration of 100mg / L;

[0016] ③ Hormone gel preparation: Accurately weigh 1g of sodium carboxymethylcellulose and 8g of sodium alginate, and dissolve them in 100mL of hormone working solution with a concentration of 100mg / L in a water bath at 50°C for later use;

[0017] Root dipping: Dip the lower end of the 8-10cm long, healthy and strong boxwood bra...

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PUM

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Abstract

The invention discloses a root dipping method of medium-concentration KT gel, and belongs to the technical field of plant biology. The method comprises the steps that KT mother liquor with the concentration being 1mg / mL is prepared at first; the mother liquor is diluted into a medium-concentration KT working solution with the concentration being 100 mg / L; the working solution is used for preparing medium-concentration KT gel confining liquid comprising sodium carboxymethylcellulose with the mass concentration being 1% and the sodium alginate with the mass concentration being 8%; the lower end of a healthy and thick branch is dipped in the gel confining liquid for 3-5 s, and then the branch is taken out; the branch is inserted into a culture substrate for rooting.

Description

technical field [0001] The invention discloses a method for dipping roots with medium-concentration KT gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as IAA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vitro propagat...

Claims

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Application Information

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IPC IPC(8): A01N25/04A01N43/90A01P21/00A01G7/06A01G1/00
Inventor 何其家
Owner 何其家